Regulated Incorporation of Two Different Metal Ions into Programmed Sites in a Duplex by DNA Polymerase Catalyzed Primer Extension

• Published in: Angewandte Chemie International Edition Vol. 53; no. 26; pp. 6624 - 6627
• Main Authors: Funai, Tatsuya, Nakamura, Junko, Miyazaki, Yuki, Kiriu, Risa, Nakagawa, Osamu, Wada, Shun-ichi, Ono, Akira, Urata, Hidehito
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 23.06.2014; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Biocatalysis; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - metabolism; DNA polymerase; DNA structures; DNA-Directed DNA Polymerase - chemistry; DNA-Directed DNA Polymerase - metabolism; Formations; Genes; Incorporation; Ions - chemistry; mercury; Metal ions; metal-mediated base pairing; Metals - chemistry; Metals - metabolism; Nucleic Acid Conformation; Nucleic acids; Nucleotides; Physical properties; Recognition; Silver; silver; DNA polymerase; DNA structures; mercury; metal-mediated base pairing
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.201311235

Metal‐mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. AgI ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C–AgI–T base pair, as well as the previously reported C–AgI–A base pair. The comparative susceptibility of dNTPs to AgI‐mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTP≫dCTP. Furthermore, two kinds of metal ions, AgI and HgII, selectively mediate the incorporation of thymidine 5′‐triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved. (Quick) silver DNA: HgII and AgI ions were found to specifically trigger DNA polymerase catalyzed primer extension in the absence of a Watson–Crick matched nucleotide through the formation of T–HgII–T and newly discovered C–AgI–T base pairs. The strict base recognition by the metal ions enabled regulated incorporation of the two different metal ions into programmed sites in duplex DNA.