Area light source‐triggered latent angiogenic molecular mechanisms intensify therapeutic efficacy of adult stem cells

• Published in: Bioengineering & translational medicine Vol. 7; no. 1; pp. e10255 - n/a
• Main Authors: Kim, Yu‐Jin, Kim, Sung‐Won, Im, Gwang‐Bum, Kim, Yeong Hwan, Jeong, Gun‐Jae, Jeon, Hye Ran, Kim, Dong‐Ik, Lee, Haeshin, Park, Sung Young, Cho, Sung Min, Bhang, Suk Ho
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.01.2022; Wiley
• Subjects: Angiogenesis; Cell adhesion & migration; Cell cycle; Cytotoxicity; Gene expression; human adipose‐derived stem cells; Irradiation; Ischemia; ischemic diseases; Kinases; latent reaction period; Light emitting diodes; Light sources; Mitochondria; Natural lighting; Physiological effects; red organic light‐emitting diode; Skin; Stains & staining; Stem cells; therapeutic angiogenesis; Tissue engineering; Transcription factors; Transplantation; Tyrosine; Vascular endothelial growth factor; red organic light‐emitting diode; therapeutic angiogenesis; human adipose‐derived stem cells; latent reaction period; ischemic diseases
• ISSN: 2380-6761; 2380-6761
• DOI: 10.1002/btm2.10255

Light‐based therapy such as photobiomodulation (PBM) reportedly produces beneficial physiological effects in cells and tissues. However, most reports have focused on the immediate and instant effects of light. Considering the physiological effects of natural light exposure in living organisms, the latent reaction period after irradiation should be deliberated. In contrast to previous reports, we examined the latent reaction period after light exposure with optimized irradiating parameters and validated novel therapeutic molecular mechanisms for the first time. we demonstrated an organic light‐emitting diode (OLED)‐based PBM (OPBM) strategy that enhances the angiogenic efficacy of human adipose‐derived stem cells (hADSCs) via direct irradiation with red OLEDs of optimized wavelength, voltage, current, luminance, and duration, and investigated the underlying molecular mechanisms. Our results revealed that the angiogenic paracrine effect, viability, and adhesion of hADSCs were significantly intensified by our OPBM strategy. Following OPBM treatment, significant changes were observed in HIF‐1α expression, intracellular reactive oxygen species levels, activation of the receptor tyrosine kinase, and glycolytic pathways in hADSCs. In addition, transplantation of OLED‐irradiated hADSCs resulted in significantly enhanced limb salvage ratio in a mouse model of hindlimb ischemia. Our OPBM might serve as a new paradigm for stem cell culture systems to develop cell‐based therapies in the future.