Using RELION software within the Scipion framework

Scipion is a modular image‐processing framework that integrates several software packages under a unified interface while taking care of file formats and conversions. Here, new developments and capabilities of the Scipion plugin for the widely used RELION software package are presented and illustrat...

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Bibliographic Details
Published inActa crystallographica. Section D, Biological crystallography. Vol. 77; no. 4; pp. 403 - 410
Main Authors Sharov, Grigory, Morado, Dustin R., Carroni, Marta, de la Rosa-Trevín, José Miguel
Format Journal Article
LanguageEnglish
Published 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.04.2021
Wiley Subscription Services, Inc
Subjects
Computer programs
cryo‐EM
image processing
Interfaces
RELION
Scipion
single‐particle analysis
Software
Software packages
User interfaces
Scipion
image processing
RELION
cryo-EM
single-particle analysis
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Summary:Scipion is a modular image‐processing framework that integrates several software packages under a unified interface while taking care of file formats and conversions. Here, new developments and capabilities of the Scipion plugin for the widely used RELION software package are presented and illustrated with an image‐processing pipeline for published data. The user interfaces of Scipion and RELION are compared and the key differences are highlighted, allowing this manuscript to be used as a guide for both new and experienced users of this software. Different on‐the‐fly image‐processing options are also discussed, demonstrating the flexibility of the Scipion framework. An overview of the Scipion plugin for the RELION software package is presented and various image‐processing capabilities within the Scipion framework are discussed.
ISSN:2059-7983
0907-4449
2059-7983
1399-0047
DOI:10.1107/S2059798321001856