High seroprevalence of caseous lymphadenitis in Brazilian goat herds revealed by Corynebacterium pseudotuberculosis secreted proteins-based ELISA

• Published in: Research in veterinary science Vol. 88; no. 1; pp. 50 - 55
• Main Authors: Seyffert, N., Guimarães, A.S., Pacheco, L.G.C., Portela, R.W., Bastos, B.L., Dorella, F.A., Heinemann, M.B., Lage, A.P., Gouveia, A.M.G., Meyer, R., Miyoshi, A., Azevedo, V.
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.02.2010; Elsevier Limited
• Subjects: animal age; Animals; Bacterial infections; Bacterial Proteins - immunology; Bank acquisitions & mergers; blood chemistry; blood serum; Brazil - epidemiology; Caseous lymphadenitis; Confidence intervals; Corynebacterium Infections - diagnosis; Corynebacterium Infections - epidemiology; Corynebacterium Infections - veterinary; Corynebacterium pseudotuberculosis; disease control; disease diagnosis; disease prevalence; ELISA; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; farm management; Female; goat diseases; Goat Diseases - diagnosis; Goat Diseases - epidemiology; Goat Diseases - microbiology; Goats; Goats - microbiology; herds; Infections; large farms; Lymphadenitis - diagnosis; Lymphadenitis - epidemiology; Lymphadenitis - microbiology; Lymphadenitis - veterinary; Male; Milk; Prevalence; protein secretion; Secreted proteins; Seroepidemiologic Studies; Seroprevalence; Veterinary medicine; Brazil; Corynebacterium pseudotuberculosis; Caseous lymphadenitis; Goats; Secreted proteins; ELISA; Seroprevalence
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2009.07.002

We conducted a seroepidemiological survey to determine the prevalence of caseous lymphadenitis (CLA) in goat herds in Minas Gerais state, Brazil. Serum samples were collected from goats ( n = 676) from 108 rural properties in 2001, covering most of the sub-regions of this ca. 586,500 square kilometer state. Antibodies against Corynebacterium pseudotuberculosis secreted proteins were detected by an indirect enzyme-linked immunosorbent assay (ELISA). Most of the animals (78.9%) tested positive for CLA; 98% of flocks presented at least one seropositive animal. Goats managed under an extensive production system had a significantly higher seroprevalence of CLA than those in intensive and semi-intensive operations. The age distribution of the animals in the flocks affected the prevalence of this disease; however, goat breed did not. We found seropositivity against C. pseudotuberculosis to be highly prevalent in these Brazilian goat herds; consequently, appropriate management practices for the control of CLA should be implemented.