Role of the ClpX from Corynebacterium crenatum involved in stress responses and energy metabolism

• Published in: Applied microbiology and biotechnology Vol. 104; no. 12; pp. 5505 - 5517
• Main Authors: Huang, Mingzhu, Zhao, Yue, Feng, Lin, Zhu, Lingfeng, Zhan, Li, Chen, Xuelan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2020; Springer; Springer Nature B.V
• Subjects: Amino acids; Applied Microbial and Cell Physiology; Bacteria; Biomedical and Life Sciences; Biotechnology; Cloning, Molecular; ClpP gene; Complementation; Corynebacterium; Corynebacterium - enzymology; Corynebacterium - genetics; Deletion mutant; Endopeptidase Clp - genetics; Endopeptidase Clp - metabolism; Energy Metabolism; Ethanol; Ethylenediaminetetraacetic acid; Fermentation; Fusion protein; Gene Expression Regulation, Bacterial; Glucose - metabolism; Industrial Microbiology; Life Sciences; Metabolism; Microbial Genetics and Genomics; Microbiology; Microorganisms; NADP; Physiological aspects; Sequence Deletion; Stress, Physiological; Stresses; China; ClpX; Industrial bacterium; ClpP; Stress responses; Energy metabolism
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-020-10597-w

ClpX and ClpP are involved in many important functions, including stress responses and energy metabolism, in microorganisms. However, the ClpX and ClpP of microbes used in industrial scale have rarely been studied. Industrial bacterial fermentation experiences a variety of stresses, and energy metabolism is extremely important for industrial bacteria. Thus, the role played by the ClpX and ClpP of industrial bacteria in fermentation should be investigated. Most microorganisms have a single clpP gene, while Corynebacterium crenatum AS 1.542 possesses two clpPs. Herein, the clpX , clpP1 , and clpP2 of C. crenatum were cloned, and its fusion protein was expressed and characterized. We also constructed clpX deletion mutant and complementation strain. Results indicate that ClpX serves an important function in thermal, pH, and ethanol stresses. It is also involved in NADPH synthesis and glucose consumption during fermentation.