A novel small compound accelerates dermal wound healing by modifying infiltration, proliferation and migration of distinct cellular components in mice

• Published in: Journal of dermatological science Vol. 74; no. 3; pp. 204 - 213
• Main Authors: Yamaoka, Hanako, Sumiyoshi, Hideaki, Higashi, Kiyoshi, Nakao, Sachie, Minakawa, Kaori, Sumida, Kayo, Saito, Koichi, Ikoma, Norihiro, Mabuchi, Tomotaka, Ozawa, Akira, Inagaki, Yutaka
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.06.2014
• Subjects: Alkadienes - pharmacology; Alkadienes - therapeutic use; Animals; Artificial dermis graft; Carrier Proteins - metabolism; Cell Movement - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Collagen Type I - genetics; Collagen Type I - metabolism; Dermatology; Drug Evaluation, Preclinical; Female; Fibroblasts - drug effects; Granulation Tissue - cytology; Humans; Keratinocytes - drug effects; Mice; Nuclear Proteins - metabolism; Pirin; Skin Ulcer - drug therapy; TGF-β/Smad3 signal; Transforming Growth Factor beta; Wound healing; Wound Healing - drug effects; hematoxylin–eosin; Wound healing; Pirin; siRNA; small interfering RNA; EGFP; Artificial dermis graft; IFN-γ; reverse transcription-polymerase chain reaction; IFN-β; enhanced green fluorescent protein; transforming growth factor-β; interferon β; RT-PCR; interferon γ; TGF-β/Smad3 signal; TGF-β; GAPDH; H–E; glyceraldehyde 3-phosphate dehydrogenase
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/j.jdermsci.2014.03.002

Abstract Background Impaired wound healing in skin ulcer is one of the major medical issues in the aged society. Wound healing is a complex process orchestrated by a number of humoral factors and cellular components. TGF-β is known to stimulate collagen production in dermal fibroblasts while inhibiting proliferation of epidermal keratinocyte. A screening of small compounds that suppress type I collagen production in fibroblasts has identified HSc025 that antagonizes the TGF-β/Smad signal. Objective We examined the effects of HSc025 on dermal wound healing and elucidated the underlying mechanisms. Methods Effects of HSc025 on the wound closure process were evaluated in a murine full-thickness excisional wound healing model. Cell proliferation and migration were estimated using primary cultures of human keratinocytes and fibroblasts. Comprehensive analyses of gene expression profiles were performed using untreated and HSc025-treated fibroblasts. Results Oral HSc025 administration suppressed macrophage infiltration and accelerated wound closure as early as at day 2 after the dermal excision. Treatment of cultured keratinocytes with HSc025 counteracted the inhibitory effects of TGF-β on cell proliferation and migration. On the other hand, HSc025 stimulated migration, but not proliferation, of dermal fibroblasts independently of TGF-β. Experiments using an artificial dermis graft revealed that HSc025 stimulated migration of collagen-producing cells into the graft tissue. A cDNA microarray analysis of untreated and HSc025-treated fibroblasts identified pirin as a critical mediator accelerating fibroblast migration. Conclusion HSc025 accelerates wound healing by modifying infiltration, proliferation and migration of distinct cellular components, which provides a novel insight into the therapy for intractable skin ulcer.