Establishment and gene expression analysis of disease-derived induced pluripotent stem cells of scleroderma

• Published in: Journal of dermatological science Vol. 84; no. 2; pp. 186 - 196
• Main Authors: Wang, Zhongzhi, PhD, Nakamura, Kayo, MD, PhD, Jinnin, Masatoshi, MD, PhD, Kudo, Hideo, MD, PhD, Goto, Mizuki, MD, PhD, Era, Takumi, MD, PhD, Kira, Tomomi, MD, PhD, Nakashima, Taiji, MD, PhD, Fukushima, Satoshi, MD, PhD, Ihn, Hironobu, MD, PhD
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2016
• Subjects: Aged; Animals; Biopsy; Calgranulin A - metabolism; CD18 Antigens - metabolism; Cell Differentiation; Collagen - metabolism; Dermatology; Fibroblasts - metabolism; Gene Expression Profiling; Humans; Induced pluripotent stem cells; Induced Pluripotent Stem Cells - cytology; Mice; Middle Aged; Phenotype; Polymerase Chain Reaction; Scleroderma, Systemic - genetics; Scleroderma, Systemic - metabolism; Sequence Analysis, RNA; Skin - metabolism; Skin - pathology; Smad6 Protein - metabolism; Systemic sclerosis; Teratoma - metabolism; Transforming Growth Factor beta2 - metabolism; Induced pluripotent stem cells; Systemic sclerosis
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/j.jdermsci.2016.08.002

Highlights • So far, there have been no reports investigating disease-derived induced pluripotent stem cells (iPSCs) of scleroderma (SSc). We recently generated iPSCs from cultured dermal fibroblasts of SSc (SSc-iPSC) to study disease mechanisms. In the present study, we have performed gene expression analysis using cultured SSc dermal fibroblasts, SSc-iPSCs, and fibroblasts re-differentiated from SSc-iPSCs.