Voltage-Seq: all-optical postsynaptic connectome-guided single-cell transcriptomics

• Published in: Nature methods Vol. 20; no. 9; pp. 1409 - 1416
• Main Authors: Csillag, Veronika, Bizzozzero, Marianne Hiriart, Noble, J. C., Reinius, Björn, Fuzik, János
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.09.2023; Nature Publishing Group
• Subjects: 631/1647/2217/2018; 631/1647/245/2225; 631/1647/334/1874/345; 631/378/340; 631/378/87; Axon guidance; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Electric potential; Electrophysiology; Gene sequencing; Genetics; Hypothalamus; Hypothalamus (ventromedial); Information processing; Life Sciences; Neurons; Onsite; Optics; Periaqueductal gray area; Proteomics; Transcriptomics; Voltage; γ-Aminobutyric acid
• ISSN: 1548-7091; 1548-7105; 1548-7105
• DOI: 10.1038/s41592-023-01965-1

Understanding the routing of neuronal information requires the functional characterization of connections. Neuronal projections recruit large postsynaptic ensembles with distinct postsynaptic response types (PRTs). PRT is typically probed by low-throughput whole-cell electrophysiology and is not a selection criterion for single-cell RNA-sequencing (scRNA-seq). To overcome these limitations and target neurons based on specific PRTs for soma harvesting and subsequent scRNA-seq, we created Voltage-Seq. We established all-optical voltage imaging and recorded the PRT of 8,347 neurons in the mouse periaqueductal gray (PAG) evoked by the optogenetic activation of ventromedial hypothalamic (VMH) terminals. PRTs were classified and spatially resolved in the entire VMH-PAG connectome. We built an onsite analysis tool named VoltView to navigate soma harvesting towards target PRTs guided by a classifier that used the VMH-PAG connectome database as a reference. We demonstrated Voltage-seq by locating VMH-driven γ-aminobutyric acid-ergic neurons in the PAG, guided solely by the onsite classification in VoltView. Voltage-Seq combines voltage imaging, optogenetics and single-cell RNA-seq for high-throughput analysis of functional and transcriptomic properties of neurons in situ.