Mapping the Segmental Microbiomes in the Human Small Bowel in Comparison with Stool: A REIMAGINE Study

• Published in: Digestive diseases and sciences Vol. 65; no. 9; pp. 2595 - 2604
• Main Authors: Leite, Gabriela G. S., Weitsman, Stacy, Parodi, Gonzalo, Celly, Shreya, Sedighi, Rashin, Sanchez, Maritza, Morales, Walter, Villanueva-Millan, Maria Jesus, Barlow, Gillian M., Mathur, Ruchi, Lo, Simon K., Jamil, Laith H., Paski, Shirley, Rezaie, Ali, Pimentel, Mark
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2020; Springer; Springer Nature B.V
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Analysis; Bacteria - classification; Bacteria - genetics; Biochemistry; Colon; Digestive system; Endoscopy; Feces - microbiology; Female; Gastroenterology; Gastrointestinal Microbiome; Hepatology; Humans; Intestine, Small - microbiology; Male; Medicine; Medicine & Public Health; Metagenomics; Microbiota; Microbiota (Symbiotic organisms); Middle Aged; Oncology; Original; Original Article; Prospective Studies; Ribonucleic acid; Ribotyping; RNA; Small intestine; Transplant Surgery; Young Adult; Jejunum; Stool; 16S metagenomic analysis; Duodenum; Microbiome
• ISSN: 0163-2116; 1573-2568; 1573-2568
• DOI: 10.1007/s10620-020-06173-x

Background Most gut microbiome studies have been performed using stool samples. However, the small intestine is of central importance to digestion, nutrient absorption, and immune function, and characterizing its microbial populations is essential for elucidating their roles in human health and disease. Aims To characterize the microbial populations of different small intestinal segments and contrast these to the stool microbiome. Methods Male and female subjects undergoing esophagogastroduodenoscopy without colon preparation were prospectively recruited. Luminal aspirates were obtained from the duodenum, jejunum, and farthest distance reached. A subset also provided stool samples. 16S rRNA sequencing was performed and analyses were carried out using CLC Genomics Workbench. Results 16S rRNA sequencing identified differences in more than 2000 operational taxonomic units between the small intestinal and stool microbiomes. Firmicutes and Proteobacteria were the most abundant phyla in the small intestine, and Bacteroidetes were less abundant. In the small intestine, phylum Firmicutes was primarily represented by lactic acid bacteria, including families Streptococcaceae, Lactobacillaceae, and Carnobacteriaceae, and Proteobacteria was represented by families Neisseriaceae, Pasteurellaceae, and Enterobacteriaceae. The duodenal and FD microbial signatures were markedly different from each other, but there were overlaps between duodenal and jejunal and between jejunal and FD microbial signatures. In stool, Firmicutes were represented by families Ruminococcaceae, Lachnospiraceae, Christensenellaceae, and Proteobacteria by class Deltaproteobacteria. Conclusions The small bowel microbiome is markedly different from that in stool and also varies between segments. These findings may be important in determining how compositional changes in small intestinal microbiota contribute to human disease states.