Identification and Characterization of a Regulated Promoter Element in the Epidermal Growth Factor Receptor Gene

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 87; no. 19; pp. 7536 - 7540
• Main Authors: Hudson, Laurie G., Thompson, Karol L., Xu, Jun, Gill, Gordon N.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.10.1990; National Acad Sciences
• Subjects: 550201 - Biochemistry- Tracer Techniques; Base Composition; Base Sequence; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; Biological and medical sciences; CARCINOGENS; Cell Nucleus - metabolism; Chromosome Deletion; Codon - genetics; Cyclic AMP - metabolism; DAYS LIVING RADIOISOTOPES; DNA; DNA probes; DNA SEQUENCING; Epidermal Growth Factor - pharmacology; ErbB Receptors - genetics; ESTERS; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; GENE REPRESSORS; GENES; Genetic mutation; Genetic vectors; GROWTH FACTORS; HELA CELLS; HeLa Cells - metabolism; Humans; ISOTOPES; LIGHT NUCLEI; MEMBRANE PROTEINS; MITOGENS; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mutation; NUCLEI; NUCLEIC ACIDS; NUCLEOPROTEINS; ODD-ODD NUCLEI; Oligonucleotide Probes; OLIGONUCLEOTIDES; ONCOGENES; ORGANIC COMPOUNDS; PHORBOL ESTERS; PHOSPHORUS 32; PHOSPHORUS ISOTOPES; Promoter regions; Promoter Regions, Genetic - drug effects; PROTEINS; RADIOISOTOPES; RECEPTORS; STRUCTURAL CHEMICAL ANALYSIS; TATA Box; Tetradecanoylphorbol Acetate - pharmacology; TRANSCRIPTION FACTORS; Regulation(control); Epidermal growth factor; Gene; Transcription promoter; DNA binding protein; Biological receptor
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.87.19.7536

We have identified a 36-base-pair proximal element (-112 to -77 relative to the AUG translation initiation codon) in the epidermal growth factor receptor 5' region that functions as a promoter; mediates inductive responses to epidermal growth factor, phorbol 12-myristate 13-acetate, and cyclic AMP; and acts in an orientation-independent manner. This region functions as an enhancer when transferred to a heterologous promoter containing a TATA box. Mutations within the 36-base-pair region alter function as assayed by reporter gene expression in recipient cells. A protein has been identified that demonstrates appropriate binding specificity to mutant DNA sequences that correlates with promoter activity observed in vivo. On the basis of DNA binding characteristics and size, the identified protein appears distinct from several previously identified transcription factors known to bind to G + C-rich regions.