Molecular cloning, tissue distribution, and pharmacological characterization of melanocortin-4 receptor in spotted scat, Scatophagus argus

• Published in: General and comparative endocrinology Vol. 230-231; pp. 143 - 152
• Main Authors: Li, Jian-Tao, Yang, Zhao, Chen, Hua-Pu, Zhu, Chun-Hua, Deng, Si-Ping, Li, Guang-Li, Tao, Ya-Xiong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2016
• Subjects: Adrenocorticotropic Hormone - metabolism; alpha-MSH - analogs & derivatives; alpha-MSH - pharmacology; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Endocrinology & Metabolism; Female; HEK293 Cells; Humans; Ligand binding; Ligands; Male; Melanocortin-4 receptor; Perciformes - genetics; Perciformes - metabolism; Phylogeny; Receptor, Melanocortin, Type 4 - genetics; Receptor, Melanocortin, Type 4 - metabolism; Scatophagus argus; Signal Transduction - drug effects; Signal Transduction - genetics; Signaling; Spotted scat; Teleostei; Tissue Distribution; NDP-MSH; ACTH; cholecystokinin; CNS; α-melanocyte stimulating hormone; agouti-related protein; cAMP; [Nle 4D-Phe 7]-α-melanocyte stimulating hormone; adrenocorticotropic hormone; MC4R; arcuate nucleus; SAMC4R; proopiomelanocortin; Scatophagus argus melanocortin-4 receptor; AgRP; α-MSH; G protein-coupled receptor; Ligand binding; N-[(3R)-1,2,3,4-tetrahydroisoquinolinium3-ylcarbonyl]-(1R)-1-(4-chlorobenzyl)-2-[4-cyclohexyl-4-(1H-1,2,4-triazol-1-ylmethyl)piperidin-1-yl]-2-oxoethylamine; melanocortin receptor; CCK; Spotted scat; central nervous system; melanocortin-4 receptor; POMC; Signaling; ARC; cyclic adenosine monophosphate; GPCR; THIQ; Tissue distribution; MCR; Melanocortin-4 receptor
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2016.04.010

Highlights • Spotted scat MC4R cDNA was cloned and phylogenetic analyses were performed. • Spotted scat mc4r was widely expressed in various peripheral tissues including gonads in sexually divergent pattern. • Spotted scat MC4R had high constitutive activity and bound to THIQ allosterically.