Inhibition of aryl hydrocarbon receptor signaling and induction of NRF2-mediated antioxidant activity by cinnamaldehyde in human keratinocytes

• Published in: Journal of dermatological science Vol. 85; no. 1; pp. 36 - 43
• Main Authors: Uchi, Hiroshi, MD, PhD, Yasumatsu, Mao, Morino-Koga, Saori, PhD, Mitoma, Chikage, MD, PhD, Furue, Masutaka, MD, PhD
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.01.2017
• Subjects: Acrolein - analogs & derivatives; Acrolein - pharmacology; Antioxidants - pharmacology; Aryl hydrocarbon receptor; Basic Helix-Loop-Helix Transcription Factors - metabolism; Benzo(a)pyrene - metabolism; Cell Line; Cinnamaldehyde; Cinnamomum aromaticum - chemistry; Dermatology; Dioxins - toxicity; Environmental Pollutants - toxicity; Epidermis - cytology; Epidermis - drug effects; Epidermis - metabolism; Heme Oxygenase-1 - metabolism; Humans; Keishibukuryogan; Keratinocytes; Keratinocytes - drug effects; Keratinocytes - metabolism; Medicine, Kampo; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; Nrf2; Oxidation-Reduction - drug effects; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Reactive Oxygen Species - metabolism; Receptors, Aryl Hydrocarbon - metabolism; RNA Interference; Signal Transduction - drug effects; 5-(and-6)-carboxy-2ʹ,7ʹ-dichlorodihydrofluorescein diacetate; dioxin responsive elements; HRP; ARNT; AHR; HO1; heme oxygenase-1; keratinocytes; benzo[ a]pyrene; spolycyclic aromatic hydrocarbons; aryl hydrocarbon receptor; BaP; carboxyH 2DCFDA; NRF2; DRE; PAH; horseradish peroxidase; ROS; reactive oxygen species; Keishibukuryogan; cinnamaldehyde; nuclear factor erythroid 2-related factor 2; AHR nuclear translocator; Cinnamaldehyde; carboxyH2DCFDA; Keratinocytes; Aryl hydrocarbon receptor; PAHs; Nrf2
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/j.jdermsci.2016.10.003

Highlights • We explore phytochemicals that inhibit AHR activation in keratinocytes. • Cinnamaldehyde contained in Cinnamomum cassia extract inhibits AHR activation. • Cinnamaldehyde activates the NRF2/HO1 pathway and alleviates ROS production. • The AHR and the NRF2/HO1 pathways are independently modulated by cinnamaldehyde. • Formulae containing Cinnamomum cassia extract inhibits AHR activation.