Efficient reduction of CO2 by the molybdenum-containing formate dehydrogenase from Cupriavidus necator (Ralstonia eutropha)

• Published in: The Journal of biological chemistry Vol. 292; no. 41; pp. 16872 - 16879
• Main Authors: Yu, Xuejun, Niks, Dimitri, Mulchandani, Ashok, Hille, Russ
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.10.2017; American Society for Biochemistry and Molecular Biology
• Subjects: BASIC BIOLOGICAL SCIENCES; biochemistry & molecular biology; biofuel; carbon dioxide; enzyme catalysis; Enzymology; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; molybdenum; multifunctional enzyme; carbon dioxide; enzyme catalysis; multifunctional enzyme; biofuel; molybdenum
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.M117.785576

The ability of the FdsABG formate dehydrogenase from Cupriavidus necator (formerly known as Ralstonia eutropha) to catalyze the reverse of the physiological reaction, the reduction of CO2 to formate utilizing NADH as electron donor, has been investigated. Contrary to previous studies of this enzyme, we demonstrate that it is in fact effective in catalyzing the reverse reaction with a kcat of 11 ± 0.4 s−1. We also quantify the stoichiometric accumulation of formic acid as the product of the reaction and demonstrate that the observed kinetic parameters for catalysis in the forward and reverse reactions are thermodynamically consistent, complying with the expected Haldane relationships. Finally, we demonstrate the reaction conditions necessary for gauging the ability of a given formate dehydrogenase or other CO2-utilizing enzyme to catalyze the reverse direction to avoid false negative results. In conjunction with our earlier studies on the reaction mechanism of this enzyme and on the basis of the present work, we conclude that all molybdenum- and tungsten-containing formate dehydrogenases and related enzymes likely operate via a simple hydride transfer mechanism and are effective in catalyzing the reversible interconversion of CO2 and formate under the appropriate experimental conditions.