A comparison of assay performance measures in screening assays: signal window, Z' factor, and assay variability ratio

In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They examine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a computer simulation, and i...

Full description

Saved in:
Bibliographic Details
Published inJournal of biomolecular screening Vol. 11; no. 3; pp. 247 - 252
Main Authors Iversen, Philip W, Eastwood, Brian J, Sittampalam, G Sitta, Cox, Karen L
Format Journal Article
LanguageEnglish
Published United States 01.04.2006
Subjects
Animals
Cell Proliferation
Lung - cytology
Lung - drug effects
Mink
Models, Theoretical
Online AccessGet full text

Cover

More Information
Summary:In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They examine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a computer simulation, and illustrate their use with example data. Based on these results, the authors recommend the Z' factor as a preferred measure of assay performance for screening assays and point out that none of these measures are adequate for characterizing concentration-response assays.
ISSN:1087-0571
2472-5552
DOI:10.1177/1087057105285610