SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand

• Published in: Nanomaterials (Basel, Switzerland) Vol. 7; no. 1; p. 8
• Main Authors: Choi, Jae Min, Hahm, Eunil, Park, Kyeonghui, Jeong, Daham, Rho, Won-Yeop, Kim, Jaehi, Jeong, Dae Hong, Lee, Yoon-Sik, Jhang, Sung Ho, Chung, Hyun Jong, Cho, Eunae, Yu, Jae-Hyuk, Jun, Bong-Hyun, Jung, Seunho
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 01.01.2017; MDPI AG
• Subjects: cyclodextrin; ethylenediamine cyclodextrin; Flavonoids; Nanomaterials; Nanostructure; Naphthalene; Organic chemistry; Raman scattering; Silicon dioxide; Silver; surface-enhanced Raman scattering (SERS); ethylenediamine cyclodextrin; surface-enhanced Raman scattering (SERS); flavonoids; cyclodextrin
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano7010008

Ethylenediamine-modified β-cyclodextrin (Et-β-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO₂@Ag@Et-β-CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-β-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO₂@Ag@Et-β-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin. In particular, luteolin was detected more strongly in the linear range 10 to 10 M than various organic molecules, namely ethylene glycol, β-estradiol, isopropyl alcohol, naphthalene, and toluene. In addition, the SERS signal for luteolin captured by the SiO₂@Ag@Et-β-CD NPs remained even after repeated washing. These results indicated that the SiO₂@Ag@Et-β-CD NPs can be used as a rapid, sensitive, and selective sensor for flavonoids.