Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity

• Published in: Biochimica et biophysica acta Vol. 1861; no. 9; pp. 1365 - 1378
• Main Authors: Abd-El-Haliem, Ahmed M., Vossen, Jack H., van Zeijl, Arjan, Dezhsetan, Sara, Testerink, Christa, Seidl, Michael F., Beck, Martina, Strutt, James, Robatzek, Silke, Joosten, Matthieu H.A.J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2016; Elsevier
• Subjects: Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Defence response; Environmental Sciences; Gene Expression Regulation, Plant; Immune receptors; Life Sciences; Lycopersicon esculentum - enzymology; Lycopersicon esculentum - genetics; Multigene Family; Phosphoinositide Phospholipase C - biosynthesis; Phosphoinositide Phospholipase C - genetics; Phosphoinositide Phospholipase C - isolation & purification; Phospholipids; Plant Immunity - genetics; PLC enzymes; Protein Kinases - genetics; Signal transduction; Vegetal Biology; PTI; PLC; InsP3; PKC; HR; TLC; Phospholipids; DAG; PI-PLC; PA; Signal transduction; At; PLC enzymes; Defence response; PIP; PAMPs; PI; Sl; ETI; Immune receptors; PIP2
• ISSN: 1388-1981; 0006-3002; 1879-2618
• DOI: 10.1016/j.bbalip.2016.01.017

Plants possess effective mechanisms to quickly respond to biotic and abiotic stresses. The rapid activation of phosphatidylinositol-specific phospholipase C (PLC) enzymes occurs early after the stimulation of plant immune-receptors. Genomes of different plant species encode multiple PLC homologs belonging to one class, PLCζ. Here we determined whether all tomato homologs encode active enzymes and whether they can generate signals that are distinct from one another. We searched the recently completed tomato (Solanum lycopersicum) genome sequence and identified a total of seven PLCs. Recombinant proteins were produced for all tomato PLCs, except for SlPLC7. The purified proteins showed typical PLC activity, as different PLC substrates were hydrolysed to produce diacylglycerol. We studied SlPLC2, SlPLC4 and SlPLC5 enzymes in more detail and observed distinct requirements for Ca2+ ions and pH, for both their optimum activity and substrate preference. This indicates that each enzyme could be differentially and specifically regulated in vivo, leading to the generation of PLC homolog-specific signals in response to different stimuli. PLC overexpression and specific inhibition of PLC activity revealed that PLC is required for both specific effector- and more general “pattern”-triggered immunity. For the latter, we found that both the flagellin-triggered response and the internalization of the corresponding receptor, Flagellin Sensing 2 (FLS2) of Arabidopsis thaliana, are suppressed by inhibition of PLC activity. Altogether, our data support an important role for PLC enzymes in plant defence signalling downstream of immune receptors. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner. •Seven genes encoding PLCs form tomato were identified.•Recombinant tomato PLC proteins showed typical PLC activity.•Tomato PLC2, PLC4 and PLC5 showed distinct requirements for Ca2+ ions and the pH.•The activity of each tomato PLC enzyme is probably differentially regulated in vivo.•The response to flg22 as well as FLS2 endocytosis are suppressed by inhibition of PLC activity.