Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia

• Published in: Balkan journal of medical genetics Vol. 23; no. 1; pp. 15 - 24
• Main Authors: Öztan, G, Aktan, M, Palanduz, S, İşsever, H, Öztürk, S, Nikerel, E, Uçur, A, Bağatir, G, Bayrak, A, Çefle, K
• Format: Journal Article
• Language: English
• Published: Sofia Sciendo 26.08.2020; De Gruyter Poland
• Subjects: Bone marrow; CDKN2A gene; Chromosome aberrations; Chronic lymphocytic leukemia; Chronic lymphocytic leukemia (CLL); Cyclin-dependent kinase 4; Cytogenetics; Fluorescence in situ hybridization; Gadd45A protein; Gene expression; Leukemia; Lymph nodes; Lymphatic leukemia; Lymphocytes B; Mcl-1 protein; MDM2 protein; Medical prognosis; Original; p53 Pathway; p53 Protein; Peripheral blood; Polymerase chain reaction; Profiler PCR (polymerase chain reaction)Array; Prognosis; Proliferating cell nuclear antigen; PTEN protein; rt2 profiler pcr (polymerase chain reaction)array; Statistical analysis
• ISSN: 1311-0160; 1311-0160; 2199-5761
• DOI: 10.2478/bjmg-2020-0007

Chronic lymphocytic leukemia (CLL) is a neoplasm characterized by excessive accumulation of B lymphocytes in the peripheral blood, bone marrow and lymph nodes. We assessed the expressions of 22 genes in the p53 pathway in 30 CLL patients and 15 healthy subjects by a RT2 Profiler PCR (polymerase chain reaction) Array technique and their relation to cytogenetic aberrations detected by fluorescent hybridization (FISH). Our Student’s -test results indicated that and BCL2 genes were statistically significant ( <0.001). For six genes and were not statistically significant. The and genes were found to be upregulated by the 2 (relative fold change in gene expression) method. The highest up-regulation was detected in and genes, 10.22- and 8.51-fold, respectively. On the other hand, the gene with a fold regulation of 1.84 was found to the highest downregulation. Overall, the and genes are related to the mechanism of the disease in the p53 pathway and may be an important predictor of the prognosis of the disease. The gene may be associated with increased risk of developing CLL. We suggest that the gene may be considered as a marker associated with CLL disease. The gene expression seems to play a protective role in CLL.