Dietary supplementation of cystinotic mice by lysine inhibits the megalin pathway and decreases kidney cystine content

• Published in: Scientific reports Vol. 13; no. 1; p. 17276
• Main Authors: Rega, L. R., Janssens, V., Graversen, J. H., Moestrup, S. K., Cairoli, S., Goffredo, B. M., Nevo, N., Courtoy, G. E., Jouret, F., Antignac, C., Emma, F., Pierreux, C. E., Courtoy, P. J.
• Format: Journal Article Web Resource
• Language: English
• Published: London Nature Publishing Group UK 12.10.2023; Nature Publishing Group; Springer Science and Business Media LLC; Nature Portfolio
• Subjects: 631/443/272; 631/80/313/1461; 692/4022/1585/1485; Accumulation; Arginine; Cystinosis; Diet; Dietary supplements; Endocytosis; Human health sciences; Humanities and Social Sciences; Kidneys; LRP2 protein; Lysine; Metabolic disorders; Multidisciplinary; Polyuria; Science; Science (multidisciplinary); Sciences de la santé humaine; Stereoisomers; Surface plasmon resonance; Urologie & néphrologie; Urology & nephrology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-43105-x

Megalin/LRP2 is a major receptor supporting apical endocytosis in kidney proximal tubular cells. We have previously reported that kidney-specific perinatal ablation of the megalin gene in cystinotic mice, a model of nephropathic cystinosis, essentially blocks renal cystine accumulation and partially preserves kidney tissue integrity. Here, we examined whether inhibition of the megalin pathway in adult cystinotic mice by dietary supplementation (5x-fold vs control regular diet) with the dibasic amino-acids (dAAs), lysine or arginine, both of which are used to treat patients with other rare metabolic disorders, could also decrease renal cystine accumulation and protect cystinotic kidneys. Using surface plasmon resonance, we first showed that both dAAs compete for protein ligand binding to immobilized megalin in a concentration-dependent manner, with identical inhibition curves by l - and d -stereoisomers. In cystinotic mice, 2-month diets with 5x- l -lysine and 5x- l -arginine were overall well tolerated, while 5x- d -lysine induced strong polyuria but no weight loss. All diets induced a marked increase of dAA urinary excretion, most prominent under 5x- d -lysine, without sign of kidney insufficiency. Renal cystine accumulation was slowed down approx. twofold by L-dAAs, and totally suppressed by d -lysine. We conclude that prolonged dietary manipulation of the megalin pathway in kidneys is feasible, tolerable and can be effective in vivo.