Sequential stimulation of cellular RNA synthesis in polyoma-infected mouse kidney cell cultures

• Published in: Nucleic acids research Vol. 11; no. 19; pp. 6611 - 6629
• Main Authors: Matter, Jean-Marc, Tiercy, Jean-Marie, Weil, Roger
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 11.10.1983
• Subjects: Animals; Biological and medical sciences; Cell Nucleolus - metabolism; Cell Nucleus - metabolism; Cell Transformation, Viral; Cells, Cultured; Fundamental and applied biological sciences. Psychology; Kidney; Mice; Microbiology; Nucleic Acid Hybridization; Nucleic Acid Precursors - genetics; Plasmids; Polyomavirus; Polyomavirus - genetics; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; RNA Precursors; RNA, Heterogeneous Nuclear - genetics; RNA, Heterogeneous Nuclear - isolation & purification; RNA, Ribosomal - genetics; RNA, Viral - genetics; Simian virus 40; Transcription, Genetic; Virology; Virus; Cell culture; Vertebrata; Mammalia; Mouse; RNA synthesis; Rodentia; Polyoma virus; Papovaviridae; Host virus relation; Hn-RNA; Kidney
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/11.19.6611

Lytic infection with polyoma virus leads in Go-arrested primary mouse kidney cell cultures to a mitotic host response. In the present work we focused our attention on cellular RNA synthesis shortly after onset of polyoma T-antigen synthesis. Onset of polyoma-induced stimulation of 45S pre-rRNA synthesis was determined by hybridization of total cellular RNA with a plasmid (pMrSalB) containing the 5'-end of the mouse ribosomal gene and of the other cellular RNA species by standard biochemical analysis of cellular fractions. The results showed that polyoma-induced stimulation of cellular hnRNA (hnRNP) synthesis, the earliest presently known host cell reaction, preceded onset of stimulated 45S pre-rRNA synthesis and that the latter was paralleled by polyoma-induced stimulation of 5S RNA, tRNA and overall protein synthesis. The polyoma-induced mitotic response is similar to that triggered by simian virus 40 and by certain nonviral mitogens.