Detection of Group C Rotavirus in Infants with Extrahepatic Biliary Atresia

• Published in: The Journal of infectious diseases Vol. 174; no. 1; pp. 8 - 15
• Main Authors: Riepenhoff-Talty, M., Gouvea, V., Evans, M. J., Svensson, L., Hoffenberg, E., Sokol, R. J., Uhnoo, I., Greenberg, S. J., Schäkel, K., Zhaori, G., Fitzgerald, J., Chong, S., El-Yousef, M., Nemeth, A., Brown, M., Piccoli, D., Hyams, J., Ruffin, D., Rossi, T.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.07.1996; University of Chicago Press; Oxford University Press
• Subjects: Autoradiography; Base Sequence; Bile Ducts, Extrahepatic - virology; Biliary atresia; Biliary Atresia - virology; Biological and medical sciences; Diarrhea; Female; Human viral diseases; Humans; Infant; Infant, Newborn; Infants; Infectious diseases; Liquids; Liver; Major Articles; Male; Medical sciences; MEDICIN; MEDICINE; Molecular Sequence Data; Polymerase chain reaction; Polymerase Chain Reaction - methods; Retrospective Studies; Reverse transcriptase polymerase chain reaction; RNA; RNA, Viral - blood; RNA-Directed DNA Polymerase; Rotavirus; Rotavirus - classification; Rotavirus - genetics; Rotavirus Infections - complications; Rotavirus Infections - diagnosis; Single-Blind Method; Viral diseases; Viral diseases of the digestive system; Viruses; Human; Rotavirus; Extrahepathic bile duct; RNA virus; Infant; Biliary tract disease; Congenital disease; Reoviridae; Infection; Virus; Association; Malformation; Viral disease; Atresia; Digestive diseases
• ISSN: 0022-1899; 1537-6613; 1537-6613
• DOI: 10.1093/infdis/174.1.8

The purpose of this retrospective study was to examine liver tissue from patients with cholestatic disease for the presence of group C rotavirus RNA. The reverse transcriptase-polymerase chain reaction (PCR) for genes 5 and 6 was used, and the PCR products were subjected to liquid hybridization with a 32P-Iabeled probe. A second amplification with nested primers was also used. Samples from 32 subjects (20 with biliary atresia or choledochal cyst and 12 controls) were tested. Ten of 20 biliary atresia patients were positive for group C rotavirus RNA; no controls were positive (P < .003). Three of the positive patients were positive for both genes 5 and 6. Six of the 10 had >1 sample that was positive. These data suggest a possible relationship between group C rotavirus and extrahepatic biliary atresia in the 10 patients in whom virus RNA was detected.