Differential host gene responses in mice infected with two highly pathogenic avian influenza viruses of subtype H5N1 isolated from wild birds in Thailand

• Published in: Virology (New York, N.Y.) Vol. 412; no. 1; pp. 9 - 18
• Main Authors: Hayashi, Tsuyoshi, Chaichoune, Kridsada, Patchimasiri, Tuangthong, Hiromoto, Yasuaki, Kawasaki, Yuri, Wiriyarat, Witthawat, Chakritbudsabong, Warunya, Prayoonwong, Natanan, Chaisilp, Natnapat, Parchariyanon, Sujira, Ratanakorn, Parntep, Uchida, Yuko, Tsuda, Tomoyuki, Saito, Takehiko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.03.2011
• Subjects: Animals; Birds; Brain - pathology; Brain - virology; Cytokine; Cytokines - biosynthesis; Disease Models, Animal; Female; Gene Expression Profiling; Highly pathogenic avian influenza virus; Host-Pathogen Interactions; Infectious Disease; Inflammation; Influenza A Virus, H5N1 Subtype - isolation & purification; Influenza A Virus, H5N1 Subtype - pathogenicity; Influenza in Birds - virology; Lung - pathology; Lung - virology; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Neutrophil; Orthomyxoviridae Infections - mortality; Orthomyxoviridae Infections - pathology; Orthomyxoviridae Infections - virology; RNA, Viral - chemistry; RNA, Viral - genetics; Sequence Analysis, DNA; Survival Analysis; Thailand; Wild bird; Thailand; Inflammation; Mice; Highly pathogenic avian influenza virus; Neutrophil; Wild bird; Cytokine
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2010.12.040

Abstract In Thailand, highly pathogenic avian influenza (HPAI) viruses of subtype H5N1 had been isolated from various wild birds during the HPAI outbreak in poultries. In this study, we examined the pathogenicity of two wild bird isolates (A/Pigeon/Thailand/VSMU-7-NPT/2004; Pigeon04 and A/Tree sparrow/Ratchaburi/VSMU-16-RBR/2005; T.sparrow05) in mice. They showed similar replication in several organs and lethal outcome. However, on day 3 post-infection, Pigeon04 induced mRNA expression of proinflammatory cytokines (IL6 and TNFα) and MIP-2, neutrophil chemoattractant, in the lungs, resulting in severe pneumonia that was accompanied by neutrophil infiltration. In contrast, on day 7 post-infection, T.sparrow05 induced the expression of several cytokines to a greater extent than Pigeon04; it also potently induced mRNA expression of several cytokines in brains of the infected mice that triggered frequent inflammatory events. In sum, our study demonstrated that two HPAI viruses induced different host responses, despite having similar replications, resulting in lethal outcome in mice.