Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants

Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of...

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Published inFrontiers in pharmacology Vol. 12; p. 653064
Main Authors Tien, Nguyen-Quang-Duc, Yang, Moon-Sik, Jang, Yong-Suk, Kwon, Tae-Ho, Reljic, Rajko, Kim, Mi-Young
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 30.04.2021
Subjects
immunity
mucosal
PEDV
Pharmacology
plant
vaccine
PEDV
vaccine
immunity
plant
mucosal
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Summary:Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.
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Reviewed by: Lijie Tang, Northeast Agricultural University, China
Hugh S. Mason, Arizona State University, United States
Faten A. Okda, St. Jude Children's Research Hospital, United States
This article was submitted to Experimental Pharmacology and Drug Discovery, a section of the journal Frontiers in Pharmacology
Edited by: Mariusz Skwarczynski, The University of Queensland, Australia
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2021.653064