Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems

• Published in: Applied microbiology and biotechnology Vol. 104; no. 13; pp. 5673 - 5688
• Main Authors: Wang, Tian-Yun, Guo, Xiao
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2020; Springer; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biotechnology; Cassettes; Cell Line; Cells; Cloning; DNA methylation; Epigenesis, Genetic; Gene Expression; Genetic Engineering; Genetic Vectors - genetics; Genomes; Health aspects; Human tissues; Humans; Life Sciences; Mammalian cells; Mammals; Microbial Genetics and Genomics; Microbiology; Mini-Review; Optimization; Protein expression; Proteins; Recombinant proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - therapeutic use; Regulatory Elements, Transcriptional; RNA polymerase; t-Plasminogen activator; Technology application; China; Cis-acting epigenetic regulatory elements; Recombinant therapeutic protein; Mammalian cell culture; Vector optimization
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-020-10640-w

Human tissue plasminogen activator was the first recombinant therapy protein that successfully produced in Chinese hamster ovary cells in 1986 and approved for clinical use. Since then, more and more therapeutic proteins are being manufactured in mammalian cells, and the technologies for recombinant protein production in this expression system have developed rapidly, with the optimization of both upstream and downstream processes. One of the most promising strategies is expression vector cassette optimization based on the expression vector cassette. In this review paper, these approaches and developments are summarized, and the future strategy on the utilizing of expression cassettes for the production of recombinant therapeutic proteins in mammalian cells is discussed.