Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

• Published in: Nature methods Vol. 20; no. 8; pp. 1174 - 1178
• Main Authors: Quardokus, Ellen M., Saunders, Diane C., McDonough, Elizabeth, Hickey, John W., Werlein, Christopher, Surrette, Christine, Rajbhandari, Presha, Casals, Anna Martinez, Tian, Hua, Lowery, Lisa, Neumann, Elizabeth K., Björklund, Frida, Neelakantan, Taruna V., Croteau, Josh, Wiblin, Anne E., Fisher, Jeremy, Livengood, April J., Dowell, Karen G., Silverstein, Jonathan C., Spraggins, Jeffrey M., Pryhuber, Gloria S., Deutsch, Gail, Ginty, Fiona, Nolan, Garry P., Melov, Simon, Jonigk, Danny, Caldwell, Michael A., Vlachos, Ioannis S., Muller, Werner, Gehlenborg, Nils, Stockwell, Brent R., Lundberg, Emma, Snyder, Michael P., Germain, Ronald N., Camarillo, Jeannie M., Kelleher, Neil L., Börner, Katy, Radtke, Andrea J.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2023; Nature Publishing Group
• Subjects: 631/1647/245/2226; 631/61; 631/80/2373; 706/648; Antibodies; Bioinformatics; Biological Microscopy; Biological Techniques; Biomarkers; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Brief Communication; Cloning; Communication; Community Resources; Construction; Costs; Data collection; Diagnostic Imaging; Humans; Imaging; Life Sciences; Lymphatic system; Lymphocytes; Mapping; Medical research; Multiplexing; Panels; Proteins; Proteomics; Reproducibility of Results; Subject specialists

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employe...