Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

• Published in: Nature methods Vol. 20; no. 8; pp. 1174 - 1178
• Main Authors: Quardokus, Ellen M., Saunders, Diane C., McDonough, Elizabeth, Hickey, John W., Werlein, Christopher, Surrette, Christine, Rajbhandari, Presha, Casals, Anna Martinez, Tian, Hua, Lowery, Lisa, Neumann, Elizabeth K., Björklund, Frida, Neelakantan, Taruna V., Croteau, Josh, Wiblin, Anne E., Fisher, Jeremy, Livengood, April J., Dowell, Karen G., Silverstein, Jonathan C., Spraggins, Jeffrey M., Pryhuber, Gloria S., Deutsch, Gail, Ginty, Fiona, Nolan, Garry P., Melov, Simon, Jonigk, Danny, Caldwell, Michael A., Vlachos, Ioannis S., Muller, Werner, Gehlenborg, Nils, Stockwell, Brent R., Lundberg, Emma, Snyder, Michael P., Germain, Ronald N., Camarillo, Jeannie M., Kelleher, Neil L., Börner, Katy, Radtke, Andrea J.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2023; Nature Publishing Group
• Subjects: 631/1647/245/2226; 631/61; 631/80/2373; 706/648; Antibodies; Bioinformatics; Biological Microscopy; Biological Techniques; Biomarkers; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Brief Communication; Cloning; Communication; Community Resources; Construction; Costs; Data collection; Diagnostic Imaging; Humans; Imaging; Life Sciences; Lymphatic system; Lymphocytes; Mapping; Medical research; Multiplexing; Panels; Proteins; Proteomics; Reproducibility of Results; Subject specialists
• ISSN: 1548-7091; 1548-7105; 1548-7105
• DOI: 10.1038/s41592-023-01846-7

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. Organ Mapping Antibody Panels are a community-led initiative to create standardized antibody panels for multiplexed spatial imaging.