Successful Transient Introduction of Leishmania RNA Virus into a Virally Infected and an Uninfected Strain of Leishmania

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 90; no. 5; pp. 1736 - 1740
• Main Authors: Armstrong, Timothy C., Keenan, Marie C., Widmer, Giovanni, Patterson, Jean L.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.03.1993; National Acad Sciences; National Academy of Sciences
• Subjects: Animal cells; Animals; Base Sequence; Biological and medical sciences; Biology; Cell Membrane Permeability; DNA; Double stranded RNA; Electric fields; Electricity; Electroporation; Fundamental and applied biological sciences. Psychology; Leishmania; Leishmania - microbiology; Microbiology; Molecular Sequence Data; Parasite hosts; Polymerase Chain Reaction; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; RNA; RNA Viruses - growth & development; RNA, Double-Stranded - analysis; Viral infections; Virology; Virus Replication; Viruses; Infection; Protozoa; Stability; Leishmania; Rhizoflagellata; Virus replication cycle; Electroporation; Replication; Infected cell; Host virus relation
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.90.5.1736

Viruses of Leishmania have recently been identified and characterized. These viruses are consistently double-stranded RNA viruses of ≈5 kb. To date, they have not been reported to exist outside their protozoan host, nor have they been shown to be infectious. We report here the ability to transiently transfer these viruses to two strains of Leishmania, one previously infected and one that did not previously carry a virus. A PCR-based assay was used to detect viral negative-stranded RNA. Input RNA was ruled out as the source of template because a replication-incompetent (UV inactivated) virus was not detectable after transfer into Leishmania.