Conditional gene manipulation： Cre-ating a new biological era

To solve the problem of embryonic lethality in conventional gene knockouts, site-specific recombinase （SSR） systems （Cre-loxP, FIp-FRT, and φC31） have been used for tissue-specific gene knockout. With the combination of an SSR system and inducible gene expression systems （tetracycline and tamoxifen）...

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Published inJournal of Zhejiang University. B. Science Vol. 13; no. 7; pp. 511 - 524
Main Authors Zhang, Jian, Zhao, Jing, Jiang, Wen-jie, Shan, Xi-wei, Yang, Xiao-mei, Gao, Jian-gang
Format Journal Article
LanguageEnglish
Published Heidelberg SP Zhejiang University Press 01.07.2012
Springer Nature B.V
Zhejiang University Press
Subjects
Biomedical and Life Sciences
Biomedicine
Biotechnology
Embryos
Gene Silencing
Gene Targeting - methods
Gene Targeting - trends
Genetic Engineering - methods
Genetic Engineering - trends
Genome, Human - genetics
Humans
Recombinases - genetics
Review
Q33
ΦC31 system
Gene targeting
Site-specific recombinase
Inducible systems
Gene trapping
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Summary:To solve the problem of embryonic lethality in conventional gene knockouts, site-specific recombinase （SSR） systems （Cre-loxP, FIp-FRT, and φC31） have been used for tissue-specific gene knockout. With the combination of an SSR system and inducible gene expression systems （tetracycline and tamoxifen）, stage-specific knockout and transgenic expression can be achieved. The application of this ＂SSR＋inducible＂ conditional tool to genomic manipulation can be extended in various ways. Alternatives to conditional gene targeting, such as conditional gene trapping, multipurpose conditional alleles, and conditional gene silencing, have been developed. SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities. With these exciting achievements, we are moving towards a new era in which the whole genome can be manipulated as we wish.
Bibliography:To solve the problem of embryonic lethality in conventional gene knockouts, site-specific recombinase （SSR） systems （Cre-loxP, FIp-FRT, and φC31） have been used for tissue-specific gene knockout. With the combination of an SSR system and inducible gene expression systems （tetracycline and tamoxifen）, stage-specific knockout and transgenic expression can be achieved. The application of this ＂SSR＋inducible＂ conditional tool to genomic manipulation can be extended in various ways. Alternatives to conditional gene targeting, such as conditional gene trapping, multipurpose conditional alleles, and conditional gene silencing, have been developed. SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities. With these exciting achievements, we are moving towards a new era in which the whole genome can be manipulated as we wish.
33-1356/Q
Site-specific recombinase, Gene targeting, Gene trapping, Inducible systems, φC31 system
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The two authors contributed equally to this work
Corresponding Authors
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1200042