Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan

• Published in: Viruses Vol. 14; no. 10; p. 2166
• Main Authors: Lal, Aamir, Kil, Eui-Joon, Vo, Thuy T. B, Wira Sanjaya, I Gusti Ngurah Prabu, Qureshi, Muhammad Amir, Nattanong, Bupi, Ali, Muhammad, Shuja, Malik Nawaz, Lee, Sukchan
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 30.09.2022; MDPI
• Subjects: begomovirus; Chenopodium album; Chenopodium leaf distortion virus; Cloning; Discovery and exploration; Enzymes; Flowers & plants; Genetic aspects; Genomes; Identification and classification; Infections; Leaf-curl; Nucleotide sequence; Open reading frames; Origin of species; Phylogenetics; Phylogeny; Plant viruses; Proteins; Recombination; Sequence analysis; Speciation; Virus diseases of plants; Virus research; Viruses; Pakistan; United States > US
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v14102166

Recombination between isolates of different virus species has been known to be one of the sources of speciation. Weeds serve as mixing vessels for begomoviruses, infecting a wide range of economically important plants, thereby facilitating recombination. Chenopodium album is an economically important weed spread worldwide. Here, we present the molecular characterization of a novel recombinant begomovirus identified from C. album in Lahore, Pakistan. The complete DNA- A genome of the virus associated with the leaf distortion occurred in the infected C. album plants was cloned and sequenced. DNA sequence analysis showed that the nucleotide sequence of the virus shared 93% identity with those of the rose leaf curl virus and the duranta leaf curl virus. Interestingly, this newly identified virus is composed of open reading frames (ORFs) from different origins. Phylogenetic networks and complementary recombination detection methods revealed extensive recombination among the sequences. The infectious clone of the newly detected virus was found to be fully infectious in C. album and Nicotiana benthamiana as the viral DNA was successfully reconstituted from systemically infected tissues of inoculated plants, thus fulfilling Koch’s postulates. Our study reveals a new speciation of an emergent ssDNA plant virus associated with C. album through recombination and therefore, proposed the tentative name ‘Chenopodium leaf distortion virus’ (CLDV).