EFFECTS OF ARILDONE ON THE IMMUNOGENICITY OF FORMALIN-INACTIVATED POLIOVIRUSES

• Published in: Japanese Journal of Medical Science and Biology Vol. 40; no. 1; pp. 1 - 13
• Main Authors: ABE, Shinobu, YAMAKI, Atsushi, DOI, Yutaka, YOSHIOKA, Isao
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 1987
• Subjects: Animals; Antigens, Viral - analysis; Antiviral Agents - pharmacology; Centrifugation, Isopycnic; Formaldehyde - pharmacology; Guinea Pigs; Ketones - pharmacology; Neutralization Tests; Poliovirus - drug effects; Poliovirus - immunology
• ISSN: 0021-5112; 1884-2828
• DOI: 10.7883/yoken1952.40.1

Concentrated and purified Sabin and virulent strains of poliovirus types 1, 2 and 3 were inactivated with formalin at 37C. By addition of 5.4μM arildone, an antiviral agent, to the virus suspension, the stability of D antigen increased in both Sabin and virulent strains of all types, especially in virulent type 1 Mahoney strain. The drug had neither any inhibitory nor enhancing effect on the formalin inactivation. When antibody response was compared in guinea pigs, Sabin strains inactivated in the absence of arildone were less immunogenic against homotypic virulent strains than inactivated vaccine prepared from virulent strains. On the other hand, Sabin strains inactivated in the presence of arildone were equally immunogenic. These results indicate that it is possible to prepare from Sabin strains a potent and safe inactivated vaccine having an immunogenicity comparable to that prepared from virulent strains.