NCAPG upregulation mediated by four microRNAs combined with activation of the p53 signaling pathway is a predictor of poor prognosis in patients with breast cancer

• Published in: Oncology letters Vol. 21; no. 4; p. 323
• Main Authors: Dong, Menglu, Xu, Tao, Cui, Xiaoqing, Li, Hanning, Li, Xingrui, Xia, Wenfei
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.04.2021; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Analysis; Breast cancer; Cancer; Cancer patients; Cell cycle; Cell division; Datasets; Development and progression; Esophagus; Gene expression; Genes; Genomics; Medical prognosis; Metastasis; MicroRNA; MicroRNAs; Mortality; Oncology, Experimental; Online databases; Pancreatic cancer; Progesterone; Prognosis; Prostate cancer; Proteins; Scientific equipment and supplies industry; Statistical analysis; Survival analysis; Tumor proteins; United States; microRNAs; breast cancer; cell division cyclin 25 homolog C; p53 signaling pathway; non-SMC condensin I complex subunit G
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2021.12585

The role of non-SMC condensin I complex subunit G (NCAPG) in breast cancer remains unclear. The present study used online databases, reverse transcription-quantitative PCR, flow cytometry and western blotting to determine the expression levels, prognosis and potential molecular mechanisms underlying the role of NCAPG in breast cancer. The association between NCAPG expression and several different clinicopathological parameters in patients with breast cancer was determined, and the results revealed that NCAPG expression was negatively associated with estrogen receptor and progesterone receptor positive status, but was positively associated with HER2 positive status, Nottingham Prognostic Index score and Scarff-Bloom-Richardson grade status. Furthermore, upregulated expression levels of NCAPG resulted in a poor prognosis in patients with breast cancer. A total of 27 microRNAs (miRNAs/miRs) were predicted to target NCAPG, among which four miRNAs (miR-101-3p, miR-195-5p, miR-214-3p and miR-944) were predicted to most likely regulate NCAPG expression in breast cancer. A total of 261 co-expressed genes of NCAPG were identified, including cell division cyclin 25 homolog C (CDC25C), and pathway enrichment analysis indicated that these co-expressed genes were significantly enriched in the p53 signaling pathway. CDC25C expression was downregulated in breast cancer and was associated with a poor prognosis. These findings suggested that upregulated NCAPG expression may be a prognostic biomarker of breast cancer.