potential strategy for high-grade gliomas: combination treatment with lithium chloride and BmK CT

• Published in: Biotechnology letters Vol. 34; no. 1; pp. 9 - 17
• Main Authors: Fu, Yuejun, Zheng, Shuhua, Huang, Rui, An, Na, Zheng, Yali, Zhang, Zhiyun, Liang, Aihua
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 2012; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Activation; Antineoplastic Agents - pharmacology; Applied Microbiology; beta Catenin - metabolism; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Buthus martensii; catalytic activity; Cell Line, Tumor; Chemotherapy; Drug Synergism; emotions; fluorescent antibody technique; Fundamental and applied biological sciences. Psychology; gelatinase A; Glioma - drug therapy; Humans; Infiltration; Life Sciences; Lithium; lithium chloride; Lithium Chloride - pharmacology; luciferase; Matrix; Matrix Metalloproteinase 2; Matrix Metalloproteinase Inhibitors; Microbiology; Moods; Organ Culture Techniques; Original Research Paper; Peptides; Proteins; Scorpion Venoms - pharmacology; Strategy; therapeutics; Therapy; Toxins; Karsch CT; β-Catenin; Glioma; Lithium chloride; Matrix metalloproteinase-2; Peptidases; Treatment; Enzyme; Buthus martensii Karsch CT; Hydrolases; Metalloendopeptidases; Gelatinase A
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-011-0741-2

Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mood stabilizer, could synergistically inhibit the migration, invasion and proliferation of C6 glioma cells. The decreased invasiveness of C6 glioma cells was accompanied by inhibited activation, catalytic activity and/or expression of matrix metalloproteinase-2. Moreover, TOPfalsh luciferase reporter and immunofluorescence staining showed altered localization pattern of β-catenin at the leading edge of 2D scratch. Our results suggested that the combination treatment of lithium and BmK CT may constitute a novel and potential strategy for HHG therapy.