Regulation of epithelial cell proliferation by bronchial fibroblasts obtained from mild asthmatic subjects

• Published in: Allergy (Copenhagen) Vol. 65; no. 11; pp. 1438 - 1445
• Main Authors: Semlali, A, Jacques, E, Rouabhia, M, Milot, J, Laviolette, M, Chakir, J
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.11.2010; Blackwell Publishing Ltd; Blackwell
• Subjects: Asthma; Asthma - immunology; Asthma - metabolism; Asthma - pathology; Biological and medical sciences; biosynthesis; Blotting, Western; Bronchi; Bronchi - immunology; Bronchi - metabolism; Bronchi - pathology; Cell Communication; Cell Communication - physiology; Cell Proliferation; Cells, Cultured; Cellular biology; Chronic obstructive pulmonary disease, asthma; Coculture Techniques; Cyclin-Dependent Kinase Inhibitor Proteins; Cyclin-Dependent Kinase Inhibitor Proteins - biosynthesis; Cyclin-Dependent Kinase Inhibitor Proteins - immunology; Dermatology; engineered bronchial tissue; Enzyme-Linked Immunosorbent Assay; epithelial cells; Epithelial Cells - immunology; Epithelial Cells - metabolism; Epithelial Cells - ultrastructure; ErbB Receptors; fibroblasts; Fibroblasts - immunology; Fibroblasts - metabolism; Fibroblasts - ultrastructure; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; immunology; Medical sciences; metabolism; Microscopy, Electron, Scanning; pathology; physiology; Pneumology; Receptor, Epidermal Growth Factor - metabolism; resident cells; Respiratory Mucosa; Respiratory Mucosa - immunology; Respiratory Mucosa - metabolism; Respiratory Mucosa - pathology; Reverse Transcriptase Polymerase Chain Reaction; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Signal Transduction; Signal Transduction - physiology; Tissue Engineering; Transforming Growth Factor beta; Transforming Growth Factor beta - biosynthesis; Transforming Growth Factor beta - immunology; ultrastructure; Human; Cell proliferation; Lung disease; Immunopathology; Respiratory disease; Dermatology; Bronchus; epithelial cells; Respiratory system; Asthma; Respiratory tract; resident cells; Tissue; Regulation(control); fibroblasts; Immunology; engineered bronchial tissue; Bronchus disease; Epithelial cell; Resident (student); Resident; Obstructive pulmonary disease; Fibroblast
• ISSN: 0105-4538; 1398-9995; 1398-9995
• DOI: 10.1111/j.1398-9995.2010.02376.x

To cite this article: Semlali A, Jacques E, Rouabhia M, Milot J, Laviolette M, Chakir J. Regulation of epithelial cell proliferation by bronchial fibroblasts obtained from mild asthmatic subjects. Allergy 2010; 65: 1438-1445. Bronchial epithelium is considered a key player in coordinating airway wall remodelling. The function of epithelial cells can be modulated by the underlying fibroblasts through autocrine and paracrine mechanisms. To investigate the effect of phenotypic changes in bronchial fibroblasts from asthmatic subjects on epithelial cell proliferation. Epithelial cells and fibroblasts derived from bronchial biopsies of asthmatic and healthy controls were cultured in an engineered model. Proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromid (MTT). Epidermal growth factor receptor (EGFR), cyclin-dependent kinase inhibitors p21 and p27 were measured by western blots. Total and active forms of transforming growth factor (TGF)-β₁ were measured using ELISA and bioassay. TGF-β was inhibited using a recombinant TGF-β soluble receptor II protein. Proliferation of epithelial cells from asthmatics (AE) is increased when cells were cultured with fibroblasts from normal controls (NF). Fibroblasts from asthmatics (AF) significantly decreased the proliferation of epithelial cells from healthy subjects (NE). Activation of p21, p27, EGFR and TGF-β₁ reflects the proliferation data by decreasing in AE cultured with NF and increasing in NE cultured with AF. Neutralization of TGF-β increased proliferation of epithelial cells cultured in the asthmatic model. Fibroblasts from asthmatic subjects regulate epithelial cell prolifearation, and TGF-β signalling may represent one of the pathway involved in these interactions.