NRAMP genes function in Arabidopsis thaliana resistance to Erwinia chrysanthemi infection

• Published in: The Plant journal : for cell and molecular biology Vol. 58; no. 2; pp. 195 - 207
• Main Authors: Segond, Diego, Dellagi, Alia, Lanquar, Viviane, Rigault, Martine, Patrit, Oriane, Thomine, Sébastien, Expert, Dominique
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.04.2009; Blackwell Publishing Ltd; Blackwell; Wiley
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis - metabolism; Arabidopsis - microbiology; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Bacteria; Bacterial plant pathogens; Biological and medical sciences; Botany; Cation Transport Proteins; Cation Transport Proteins - genetics; Cation Transport Proteins - metabolism; DNA, Plant; DNA, Plant - genetics; Erwinia chrysanthemi; Ferritins; Ferritins - genetics; Ferritins - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genes, Plant; Genetics; Immunity, Innate; Iron; Iron - deficiency; Life Sciences; Metals; Mutation; NRAMP; Oligonucleotide Array Sequence Analysis; Oxidative Stress; Pathology. Damages, economic importance; Pectobacterium chrysanthemi; Pectobacterium chrysanthemi - growth & development; Phytopathology. Animal pests. Plant and forest protection; plant defense; Plant Leaves; Plant Leaves - genetics; Plant Leaves - immunology; Plant Leaves - metabolism; Plant Leaves - microbiology; Plant pathology; Plant Roots; Plant Roots - genetics; Plant Roots - immunology; Plant Roots - metabolism; Plants, Genetically Modified; Plants, Genetically Modified - genetics; Plants, Genetically Modified - immunology; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - microbiology; Pseudomonas syringae; Vegetal Biology; Oxidative stress; Arabidopsis; Biological transport; Host agent relation; Iron; Transport process; Arabidopsis thaliana; Infection; Resistance; NRAMP; plant defense; Gene; Cruciferae; Dicotyledones; Angiospermae; Erwinia chrysanthemi; Bacteria; Defense mechanism; Spermatophyta; Enterobacteriaceae; OXIDATIVE STRESS; IRON; ARABIDOPSIS; PLANT DEFENSE; ERWINIA CHRYSANTHEMI
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/j.1365-313X.2008.03775.x

AtNRAMP3 and AtNRAMP4 are two Arabidopsis metal transporters sharing about 50% sequence identity with mouse NRAMP1. The NRAMP1/Slc11A1 metal ion transporter plays a crucial role in the innate immunity of animal macrophages targeted by intracellular bacterial pathogens. AtNRAMP3 and AtNRAMP4 localize to the vacuolar membrane. We found that AtNRAMP3 is upregulated in leaves challenged with the bacterial pathogens Pseudomonas syringae and Erwinia chrysanthemi, whereas AtNRAMP4 expression is not modified. Using single and double nramp3 and nramp4 mutants, as well as lines ectopically expressing either of these genes, we show that AtNRAMP3 and, to a lesser extent, AtNRAMP4 are involved in Arabidopsis thaliana resistance against the bacterial pathogen E. chrysanthemi. The susceptibility of the double nramp3 nramp4 mutant is associated with the reduced accumulation of reactive oxygen species and ferritin (AtFER1), an iron storage protein known to participate in A. thaliana defense. Interestingly, roots from infected plants accumulated transcripts of AtNRAMP3 as well as the iron-deficiency markers IRT1 and FRO2. This finding suggests the existence of a shoot-to-root signal reminiscent of an iron-deficiency signal activated by pathogen infection. Our data indicate that the functions of NRAMP proteins in innate immunity have been conserved between animals and plants.