Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide

• Published in: Asian Pacific journal of tropical biomedicine Vol. 1; no. 1; pp. 57 - 63
• Main Authors: Garg Neeraj, K, Sharad, Mangal, Tejram, Sahu, Abhinav, Mehta, Vyas Suresh, P, Tyagi Rajeev, K
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 2011; Asian Pacific Tropical Medicine Press
• Subjects: Anticancer; Antioxidants; Antioxidants - pharmacology; Apoptosis; Apoptosis - drug effects; Carcinoma, Renal Cell; Cell Line, Tumor; Cell Survival - drug effects; DNA Fragmentation - drug effects; Humans; Hydrogen; Hydrogen peroxide; Hydrogen Peroxide - toxicity; Original; Oxidative Stress - drug effects; peroxide; RCC-26; Reactive Oxygen Species - analysis; Reactive Oxygen Species - metabolism; ROS; Vitamins - pharmacology; Antioxidants; Hydrogen peroxide; RCC-26; Anticancer; ROS; Apoptosis
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/s2221-1691(11)60069-5

Objective:To evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid,a-tocopherol acetate,citric acid,salicylic acid,and estimate H2O2induced apoptosis in renal cell carcinoma cells.Methods:The intracellular antioxidant potency of antioxidants was investigated.H2O-2-induced apoptosis in RCC-26 was assayed with the following parameters:cell viability（%apoptosis）,nucleosomal damage and DNA fragmentation, bcl-2 levels and flow cytometery analysis（ROS production evaluation）.Results:Ine anticancer properties of antioxidants such as ascorbic acid,a- tocopherol acetate,citric acid,salicylic acid with perdurable responses were investigated.It was observed that these antioxidants had protective effect（anti-apoptotic activity） against hydrogen peroxide（H2O2） in renal cell carcinoma（RCC-26） cell line.Conclusions:This study reveals and proves the anticancer properties.However,in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging,and significantly protect nucleus and bcl-2.