A conserved ATG2‐GABARAP family interaction is critical for phagophore formation

• Published in: EMBO reports Vol. 21; no. 3; pp. e48412 - n/a
• Main Authors: Bozic, Mihaela, van den Bekerom, Luuk, Milne, Beth A, Goodman, Nicola, Roberston, Lisa, Prescott, Alan R, Macartney, Thomas J, Dawe, Nina, McEwan, David G
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.03.2020; Blackwell Publishing Ltd; John Wiley and Sons Inc
• Subjects: Accumulation; Apoptosis Regulatory Proteins - metabolism; ATG2; autophagosome; Autophagosomes - metabolism; Autophagy; Autophagy-Related Proteins - genetics; Autophagy-Related Proteins - metabolism; Conserved sequence; Elongation; EMBO07; GABARAP; GABARAP protein; Homeostasis; Humans; Lysosomes; Membrane Proteins - metabolism; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Mutation; Phagocytosis; phagophore; Protein Transport; Proteins; Vesicular Transport Proteins - metabolism; ATG2; autophagy; autophagosome; GABARAP; phagophore
• ISSN: 1469-221X; 1469-3178
• DOI: 10.15252/embr.201948412

The intracellular trafficking pathway, macroautophagy, is a recycling and disposal service that can be upregulated during periods of stress to maintain cellular homeostasis. An essential phase is the elongation and closure of the phagophore to seal and isolate unwanted cargo prior to lysosomal degradation. Human ATG2A and ATG2B proteins, through their interaction with WIPI proteins, are thought to be key players during phagophore elongation and closure, but little mechanistic detail is known about their function. We have identified a highly conserved motif driving the interaction between human ATG2 and GABARAP proteins that is in close proximity to the ATG2‐WIPI4 interaction site. We show that the ATG2A‐GABARAP interaction mutants are unable to form and close phagophores resulting in blocked autophagy, similar to ATG2A/ATG2B double‐knockout cells. In contrast, the ATG2A‐WIPI4 interaction mutant fully restored phagophore formation and autophagy flux, similar to wild‐type ATG2A. Taken together, we provide new mechanistic insights into the requirements for ATG2 function at the phagophore and suggest that an ATG2‐GABARAP/GABARAP‐L1 interaction is essential for phagophore formation, whereas ATG2‐WIPI4 interaction is dispensable. Synopsis A direct interaction between ATG2A and GABARAP family of proteins is essential for phagophore maturation. Loss of this interaction results in the accumulation of open and immature vesicles and complete blockage of autophagy flux. ATG2 proteins contain a highly conserved LC3 interaction region (LIR) in close proximity to their WIPI4 interaction motif. Mutation of the ATG2A‐LIR, but not the WIPI4 interaction, is sufficient to block autophagy. ATG2A‐LIR mutant results in the accumulation of immature and open phagophores similar to those observed in ATG2A/ATG2B double knockout cells. Graphical Abstract A direct interaction between ATG2A and GABARAP family of proteins is essential for phagophore maturation. Loss of this interaction results in the accumulation of open and immature vesicles and complete blockage of autophagy flux.