The transcriptome‐wide landscape of molecular subtype‐specific mRNA expression profiles in acute myeloid leukemia

• Published in: American journal of hematology Vol. 96; no. 5; pp. 580 - 588
• Main Authors: Mou, Tian, Pawitan, Yudi, Stahl, Matthias, Vesterlund, Mattias, Deng, Wenjiang, Jafari, Rozbeh, Bohlin, Anna, Österroos, Albin, Siavelis, Loannis, Bäckvall, Helena, Erkers, Tom, Kiviluoto, Santeri, Seashore‐Ludlow, Brinton, Östling, Päivi, Orre, Lukas M., Kallioniemi, Olli, Lehmann, Sören, Lehtiö, Janne, Vu, Trung Nghia
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.05.2021; Wiley Subscription Services, Inc
• Subjects: Acute myeloid leukemia; Biomarkers, Tumor; Gene expression; Genes, Neoplasm; Genomes; Hematology; Humans; Leukemia; Leukemia, Myeloid, Acute - classification; Leukemia, Myeloid, Acute - genetics; Leukemia, Myeloid, Acute - metabolism; Medicin och hälsovetenskap; Myeloid leukemia; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Oncogene Proteins, Fusion - biosynthesis; Oncogene Proteins, Fusion - genetics; Proteome; Proteomics; Retrospective Studies; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; RNA, Neoplasm - biosynthesis; RNA, Neoplasm - genetics; RNA-Seq; Sweden; Transcriptome; Transcriptomes; Sweden
• ISSN: 0361-8609; 1096-8652; 1096-8652
• DOI: 10.1002/ajh.26141

Molecular classification of acute myeloid leukemia (AML) aids prognostic stratification and clinical management. Our aim in this study is to identify transcriptome‐wide mRNAs that are specific to each of the molecular subtypes of AML. We analyzed RNA‐sequencing data of 955 AML samples from three cohorts, including the BeatAML project, the Cancer Genome Atlas, and a cohort of Swedish patients to provide a comprehensive transcriptome‐wide view of subtype‐specific mRNA expression. We identified 729 subtype‐specific mRNAs, discovered in the BeatAML project and validated in the other two cohorts. Using unique proteomics data, we also validated the presence of subtype‐specific mRNAs at the protein level, yielding a rich collection of potential protein‐based biomarkers for the AML community. To enable the exploration of subtype‐specific mRNA expression by the broader scientific community, we provide an interactive resource to the public.