OPACIFICATION TEST BY USING THE PIG ISOLATED CORNEA AND ITS APPLICATION TO A TEST OF CORNEAL OPACITY INDUCED BY BEFUNOLOL HYDROCHLORIDE

• Published in: Journal of toxicological sciences Vol. 14; no. 2; pp. 91 - 103
• Main Authors: IGARASHI, Haruyoshi, KATSUTA, Yasunaga, MATSUNO, Hidefumi, NAKAZATO, Yoshiharu, KAWASAKI, Tohru
• Format: Journal Article
• Language: English
• Published: Tokyo The Japanese Society of Toxicology 1989; Japanese Society of Toxicology; Japan Science and Technology Agency
• Subjects: Adrenergic beta-Antagonists - toxicity; Animals; befunolol hydrochloride; Biological and medical sciences; Corneal Opacity - chemically induced; Dose-Response Relationship, Drug; Drug toxicity and drugs side effects treatment; Epithelium - physiology; In Vitro Techniques; Medical sciences; Miscellaneous (drug allergy, mutagens, teratogens...); Pharmacology. Drug treatments; Propanolamines - administration & dosage; Propanolamines - toxicity; Swine; Cornea; Toxicity; In vitro; Isolated organ; Pig; Vertebrata; Eye disease; Mammalia; Investigation method; Animal; Artiodactyla; Opacity; Ungulata; Beta blocking agent
• ISSN: 0388-1350; 1880-3989
• DOI: 10.2131/jts.14.91

Corneas isolated from the pig eyeball have been treated in vitro with varying concentrations of befunolol hydrochloride, in order to test the topical effect of befunolol on the cornea in terms of opacity. Experiments were carried out with the corneas treated as follows : intact, epithelium-removed. endothelium-removed, and both epithelium- and endothelium-removed. Solutions of the drug were applied to both epithelial and endothelidl surfaces, to the epithelial surface only, or to the endothelial surface only. When the drug was applied either to both surfaces or to the endothelial surface only, there was a significant increase in opacity. However, when applied to the epithelial surface only, befunolol caused an insignificant increase in opacity as compared with that of control. It is suggested, therefore, that corneal opacity due to befunolol hydrochloride topically administered to the eye, is concerned mainly with the epithelium in cornea, since the drug is uncapable of passing barrier probably existing in surface layer of epithelium but it easily penetrates in corneal layers from endothelial side to attain reversely to epithelial cells. Thus, it is probable that the risk to induce opacity is minor in cade that befunolol is topically given in drops to the eye.