The Acute Effects of a Single Dose of Molecular Hydrogen Supplements on Responses to Ergogenic Adjustments during High-Intensity Intermittent Exercise in Humans

• Published in: Nutrients Vol. 14; no. 19; p. 3974
• Main Authors: Alharbi, Ahad Abdulkarim D, Iwamoto, Noriaki, Ebine, Naoyuki, Nakae, Satoshi, Hojo, Tatsuya, Fukuoka, Yoshiyuki
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 24.09.2022; MDPI
• Subjects: acid status; Acid-base status; Acidosis; Acute effects; Blood flow; Body weight; Caffeine; Deoxygenation; Electrolytes; Exercise; exercise performance; Experiments; Heart rate; Hemoglobin; high intensity exercise; Hydrogen; Infrared spectra; Infrared spectroscopy; Measurement techniques; Metabolism; Microvasculature; muscle deoxygenation; Muscles; Near infrared radiation; peak power; Physical fitness; Placebos; Potassium; Workloads; Japan
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14193974

This research examined the effects of single-dose molecular hydrogen (H2) supplements on acid-base status and local muscle deoxygenation during rest, high-intensity intermittent training (HIIT) performance, and recovery. Ten healthy, trained subjects in a randomized, double-blind, crossover design received H2-rich calcium powder (HCP) (1500 mg, containing 2.544 μg of H2) or H2-depleted placebo (1500 mg) supplements 1 h pre-exercise. They performed six bouts of 7 s all-out pedaling (HIIT) at 7.5% of body weight separated by 40 s pedaling intervals, followed by a recovery period. Blood gases’ pH, PCO2, and HCO3− concentrations were measured at rest. Muscle deoxygenation (deoxy[Hb + Mb]) and tissue O2 saturation (StO2) were determined via time-resolved near-infrared spectroscopy in the vastus lateralis (VL) and rectus femoris (RF) muscles from rest to recovery. At rest, the HCP group had significantly higher PCO2 and HCO3− concentrations and a slight tendency toward acidosis. During exercise, the first HIIT bout’s peak power was significantly higher in HCP (839 ± 112 W) vs. Placebo (816 ± 108 W, p = 0.001), and HCP had a notable effect on significantly increased deoxy[Hb + Mb] concentration during HIIT exercise, despite no differences in heart rate response. The HCP group showed significantly greater O2 extraction in VL and microvascular (Hb) volume in RF during HIIT exercise. The HIIT exercise provided significantly improved blood flow and muscle reoxygenation rates in both the RF and VL during passive recovery compared to rest in all groups. The HCP supplement might exert ergogenic effects on high-intensity exercise and prove advantageous for improving anaerobic HIIT exercise performance.