A new positive-strand RNA virus with unique genome characteristics from the red imported fire ant, Solenopsis invicta
Abstract We report the discovery of a new virus with unique genome characteristics from the red imported fire ant, Solenopsis invicta . This virus represents the second identified from this ant species. It is provisionally named Solenopsis invicta virus 2 (SINV-2). The SINV-2 genome was constructed...
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Published in | Virology (New York, N.Y.) Vol. 365; no. 2; pp. 457 - 463 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.09.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Abstract We report the discovery of a new virus with unique genome characteristics from the red imported fire ant, Solenopsis invicta . This virus represents the second identified from this ant species. It is provisionally named Solenopsis invicta virus 2 (SINV-2). The SINV-2 genome was constructed by compiling sequences from successive 5′ RACE reactions, a 3′ RACE reaction, and expressed sequence tag, c246 (accession number EH413675 ), from a fire ant expression library. The SINV-2 genome structure was monopartite, polycistronic and RNA-based. The genome consensus sequence ( EF428566 ) was 11,303 nucleotides in length, excluding the poly(A) tail present on the 3′ end. Analysis of the genome revealed 4 major open reading frames (ORFs; comprised of ≥ 100 codons) and 5 minor ORFs (comprised of 50–99 codons) in the sense orientation. No large ORFs were found in the inverse orientation suggesting that the SINV-2 genome was from a positive-strand RNA virus. Further evidence for this conclusion includes abolished RT–PCR amplification by RNase treatment of SINV-2 nucleic acid template, and failure to amplify without first conducting cDNA synthesis. Blastp analysis indicated that ORF 4 contained conserved domains of an RNA-dependent RNA polymerase, helicase, and protease, characteristic of positive-strand RNA viruses. However, the protease domain and putative structural proteins (ORFs 1, 2, and 3) were less well conserved. Phylogenetic analysis of the RdRp, helicase, and ORF 1 indicate unique placement of SINV-2 exclusive from the Dicistroviridae , iflaviruses, Picornaviridae , and plant small RNA viruses. |
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Bibliography: | http://dx.doi.org/10.1016/j.virol.2007.03.043 http://hdl.handle.net/10113/7582 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0042-6822 1096-0341 |
DOI: | 10.1016/j.virol.2007.03.043 |