Cloning, expression and evaluation of the efficacy of a recombinant Eimeria tenella sporozoite antigen in birds

• Published in: Vaccine Vol. 26; no. 27; pp. 3489 - 3496
• Main Authors: Mohana Subramanian, B, Sriraman, R, Hanumantha Rao, N, Raghul, J, Thiagarajan, D, Srinivasan, V.A
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 25.06.2008; Elsevier
• Subjects: Allergy and Immunology; Amino Acid Sequence; Animals; Antibodies, Protozoan - blood; Antigens, Protozoan - genetics; Antigens, Protozoan - immunology; Applied microbiology; Aves; Biological and medical sciences; Bird Diseases - immunology; Bird Diseases - prevention & control; Cells, Cultured; Chickens; Cloning, Molecular; Coccidiosis; Coccidiosis - immunology; Coccidiosis - prevention & control; Coccidiosis - veterinary; Eimeria tenella; Eimeria tenella - genetics; Eimeria tenella - isolation & purification; Fundamental and applied biological sciences. Psychology; Gene Expression; Human protozoal diseases; IFN-γ; India; Infectious diseases; Interferon-gamma - biosynthesis; Leukocytes, Mononuclear - immunology; Male; Medical sciences; Microbiology; Microneme-1; Molecular Sequence Data; Parasitic diseases; Poultry disease; Protozoal diseases; Protozoan Infections, Animal - immunology; Protozoan Infections, Animal - prevention & control; Protozoan Vaccines - immunology; Sequence Analysis, DNA; Spleen - immunology; Sub-unit vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); India; IFN-γ; Sub-unit vaccine; Poultry disease; Coccidiosis; Microneme-1; Protozoa; Apicomplexa; Protozoal disease; Farming animal; Endoparasite; Poultry; Cytokine; Eimeria tenella; Vaccine; Parasitosis; Infection; Vertebrata; Efficiency; Recombinant protein; Veterinary; Aves; Gamma interferon
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2008.04.024

Abstract Eimeria infection in poultry is of significant economic interest worldwide. Development of a cost-effective sub-unit vaccine that provides cross-protection may help reduce loss in poultry industry. One approach explored by many investigators is to block the parasite invasion into gut epithelium. Use of microneme proteins to prevent parasite invasion is one of the most straightforward approaches in developing a preventive vaccine. Here we describe cloning and expression of microneme-1 protein of Eimeria tenella , obtained from an outbreak sample from India. We have evaluated the ability of the recombinant protein to elicit both cell mediated immune (CMI) and humoral immune responses. We also evaluated the efficacy of the recombinant protein in protecting against a homologous challenge. Our data indicate recombinant EtMIC1 is able to impart partial protection against homologous challenge in chicken. Inclusion of more invasion proteins may improve the efficacy of prophylactic vaccine against Coccidiosis.