Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes

• Published in: Nature communications Vol. 11; no. 1; pp. 5018 - 11
• Main Authors: Brouwer, Stephan, Barnett, Timothy C., Ly, Diane, Kasper, Katherine J., De Oliveira, David M. P., Rivera-Hernandez, Tania, Cork, Amanda J., McIntyre, Liam, Jespersen, Magnus G., Richter, Johanna, Schulz, Benjamin L., Dougan, Gordon, Nizet, Victor, Yuen, Kwok-Yung, You, Yuanhai, McCormick, John K., Sanderson-Smith, Martina L., Davies, Mark R., Walker, Mark J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.10.2020; Nature Portfolio
• Subjects: 13/1; 13/106; 14/34; 14/63; 38/1; 38/22; 38/39; 38/70; 38/77; 38/91; 631/326/1320; 64/110; 692/420/254; 82/29; 82/80; 82/83; Animals; Bacterial Proteins - pharmacology; Cell Line; Erythrocytes - drug effects; Exotoxins - genetics; Exotoxins - metabolism; Female; Glutathione - metabolism; Humanities and Social Sciences; Humans; Male; Mice, Inbred C57BL; Mice, Transgenic; multidisciplinary; Mutation; Pharynx - cytology; Prophages - genetics; Scarlet Fever - epidemiology; Scarlet Fever - microbiology; Science; Science (multidisciplinary); Streptococcus pyogenes - genetics; Streptococcus pyogenes - pathogenicity; Streptococcus pyogenes - virology; Streptolysins - pharmacology; Superantigens - genetics; Superantigens - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-18700-5

The re-emergence of scarlet fever poses a new global public health threat. The capacity of North-East Asian serotype M12 ( emm 12) Streptococcus pyogenes (group A Streptococcus , GAS) to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir encoding the secreted superantigens SSA and SpeC and the DNase Spd1. Here, we report the molecular characterization of ΦHKU.vir-encoded exotoxins. We demonstrate that streptolysin O (SLO)-induced glutathione efflux from host cellular stores is a previously unappreciated GAS virulence mechanism that promotes SSA release and activity, representing the first description of a thiol-activated bacterial superantigen. Spd1 is required for resistance to neutrophil killing. Investigating single, double and triple isogenic knockout mutants of the ΦHKU.vir-encoded exotoxins, we find that SpeC and Spd1 act synergistically to facilitate nasopharyngeal colonization in a mouse model. These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins. The pathogenesis of Streptococcus pyogenes (GAS) causing scarlet fever has been associated with the presence of prophages, such as ΦHKU.vir, and their products. Here, the authors characterize the exotoxins SpeC and Spd1 of ΦHKU.vir and show these to act synergistically to facilitate nasopharyngeal colonization in mice.