Bioreactors with immobilized lipases: State of the art

• Published in: Enzyme and Microbial Technology Vol. 18; no. 6; pp. 392 - 416
• Main Authors: Balcão, Victor M., Paiva, Ana L., Xavier Malcata, F.
• Format: Book Review Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.05.1996; Elsevier Science
• Subjects: activity; Amino Acid Sequence; Animals; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Biological and medical sciences; Bioreactors; Biotechnology; Catalysis; Chemical Industry - instrumentation; Chemical Industry - methods; Consensus Sequence; Detergents; Emulsions; Enzymes, Immobilized; Esterification; Fundamental and applied biological sciences. Psychology; Fungal Proteins - chemistry; Fungal Proteins - metabolism; Hydrolysis; Immobilized lipases; Lipase - chemistry; Lipase - metabolism; Methods. Procedures. Technologies; Molecular Sequence Data; optimum operating conditions; Protein Engineering; reactors; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; stability; Stereoisomerism; Structure-Activity Relationship; Various methods and equipments; optimum operating conditions; reactors; activity; stability; Immobilized lipases; State of the art; Stability; Enzyme; Industrial application; Immobilization; Triacylglycerol lipase; Environmental factor; Esterases; Review; Method; Optimization; Carboxylic ester hydrolases; Bioreactor; Enzymatic activity; Adsorption; Biotransformation; Hydrolases; Immobilized enzyme
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/0141-0229(95)00125-5

This review attempts to provide an updated compilation of studies reported in the literature pertaining to reactors containing lipases in immobilized forms, in a way that helps the reader direct a bibliographic search and develop an integrated perspective of the subject. Highlights are given to industrial applications of lipases (including control and economic considerations), as well as to methods of immobilization and configurations of reactors in which lipases are used. Features associated with immobilized lipase kinetics such as enzyme activities, adsorption properties, optimum operating conditions, and estimates of the lumped parameters in classical kinetic formulations (Michaelis-Menten model for enzyme action and first-order model for enzyme decay) are presented in the text in a systematic tabular form.