IncP-1β Plasmids Are Important Carriers of Fitness Traits for Variovorax Species in the Mycosphere—Two Novel Plasmids, pHB44 and pBS64, with Differential Effects Unveiled

• Published in: Microbial ecology Vol. 70; no. 1; pp. 141 - 153
• Main Authors: Zhang, Miaozhi, Warmink, Jan, Pereira e Silva, Michele C., Brons, Jolanda, Smalla, Kornelia, van Elsas, Jan Dirk
• Format: Journal Article
• Language: English
• Published: New York Springer 01.07.2015; Springer US
• Subjects: Analysis of Variance; Biofilms - growth & development; Biomedical and Life Sciences; Blotting, Southern; Comamonadaceae - genetics; Comamonadaceae - physiology; Ecology; ENVIRONMENTAL MICROBIOLOGY; Escherichia coli; Geoecology/Natural Processes; Karsten; Laccaria; Life Sciences; Microbial Ecology; Microbiology; Nature Conservation; Plasmids - genetics; Polymerase Chain Reaction - methods; Population Dynamics; Pseudomonas fluorescens; Replicon - genetics; Soil Microbiology; Variovorax; Variovorax paradoxus; Water Quality/Water Pollution; Plasmid; Mycosphere; Iron acquisition; IncP-1 β; Horizontal gene transfer
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-014-0550-y

The Laccaria proxima mycosphere strongly selects Variovorax paradoxus cells. Fifteen independent V. paradoxus strains, isolated from mycospheres sampled at two occasions, were investigated with respect to the occurrence of plasmids of sizes <60–100 kb. Two V. paradoxus strains, HB44 and BS64, were found to contain such plasmids, which were coined pHB44 and pBS64. Replicon typing using a suite of plasmid-specific PCR systems indicated that both plasmids belong to the IncP-1β group. Also, both were able to mobilize selectable IncQ group plasmids into Escherichia coli as well as Pseudomonas fluorescens. Moreover, they showed stable replication in these organisms, confirming their broad host range. Strain BS64 was cured of pBS64 and plasmid pHB44 was subsequently moved into this cured strain by making use of the IncQ group tracer plasmid pSUP104, which was then removed at elevated temperature. Thus, both plasmids could be screened for their ability to confer a phenotype upon strain BS64. No evidence for the presence of genes for xenobiotic degradation and/or antibiotic or heavy metal resistances was found for either of the two plasmids. Remarkably, both could stimulate the production of biofilm material by strain BS64. Also, the population densities of pBS64-containing strain BS64 were temporarily raised in liquid as well as soil systems (versus the plasmid-cured strain), both in the presence of the fungal host Lyophyllum sp. strain Karsten. Strikingly, plasmid pHB44 significantly enhanced the fitness of strain BS64 in soil containing Lyophyllum sp. strain Karsten, but decreased its fitness in soil supplemented with extra FeCl3. The effect was noted both in separate (no inter-strain competition) and joint (competition) inoculations.