In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology Vol. 158; no. 2; pp. 64 - 71
• Main Authors: Hulak, Martin, Gazo, Ievgeniia, Shaliutina, Anna, Linhartova, Pavla
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2013
• Subjects: Acipenser ruthenus; adenosine triphosphate; Adenosine Triphosphate - metabolism; adverse effects; Animals; Antioxidants - metabolism; Benzhydryl Compounds - toxicity; bisphenol A; Comet assay; DNA; DNA - genetics; DNA fragmentation; DNA Fragmentation - drug effects; Endocrine Disruptors - toxicity; endocrine-disrupting chemicals; fish; Fishes - genetics; humans; Lipid oxidation; lipid peroxidation; Male; Olea; oxidative stress; Oxidative Stress - drug effects; Phenols - toxicity; reproductive performance; sperm concentration; sperm motility; Sperm Motility - drug effects; spermatozoa; Spermatozoa - drug effects; Spermatozoa - metabolism; Spermatozoa motility; Sterlet sperm; superoxide dismutase; BPA; LO; Sterlet sperm; Lipid oxidation; SOD; TBARS; CP; Comet assay; Spermatozoa motility
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2013.05.002

Among endocrine disruptors, the xenoestrogen bisphenol A (BPA) deserves particular attention due to widespread human exposure. Besides hormonal effects, BPA has been suspected to be responsible for adverse effect on reproductive ability of various species. In the present study the effect of BPA on the quality parameters, oxidative stress, the DNA integrity and intracellular ATP content of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were exposed to concentrations of BPA possibly occurring in nature (0.5, 1.75, 2.5, 5 and 10μg/L) for 2h. Results revealed that BPA significantly decreased spermatozoa motility and velocity of spermatozoa at concentration of BPA 2.5–10μg/L. Significant positive correlation (r=0.713, P<0.05) was found between percent motile spermatozoa and ATP content. Oxidative stress was observed at concentrations 1.75–10μg/L, as reflected by significantly higher levels of protein and lipid oxidation and superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of BPA. A dramatic increase in DNA fragmentation expressed as percent tail DNA (2.2%±0.46) and Olive tail moment (0.37±0.09 arbitrary units) was recorded at concentrations of 1.75μg/L and above. The present study confirms that concentrations of BPA that can be encountered in nature are capable to induce oxidative stress, leading to impaired sperm quality, DNA fragmentation and intracellular ATP content.