Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches

• Published in: Bioprocess and biosystems engineering Vol. 38; no. 2; pp. 299 - 305
• Main Authors: Kang, In Yeong, Park, Jang Min, Hong, Won-Kyung, Kim, Yun Seok, Jung, You Ree, Kim, Seung-Bum, Heo, Sun-Yeon, Lee, Sung-Mok, Kang, Ji Young, Oh, Baek-Rock, Kim, Dae-Hyuk, Seo, Jeong-Woo, Kim, Chul Ho
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2015; Springer Nature B.V
• Subjects: Alcohol Oxidoreductases - genetics; Alcohol Oxidoreductases - metabolism; Arecaceae - microbiology; Bacillus; Bacillus - classification; Bacillus - physiology; Bacteria; Biotechnology; Butylene Glycols - isolation & purification; Butylene Glycols - metabolism; Chemistry; Chemistry and Materials Science; E coli; Environmental Engineering/Biotechnology; Enzymatic activity; Escherichia coli; Food Science; Fruit - microbiology; Fruits; Genetic engineering; Genetic Enhancement - methods; Hydrolysis; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Original Paper; Species Specificity; 2,3-butanediol dehydrogenase; Empty palm fruit bunches; sp; 2,3-butanediol
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-014-1268-4

A Bacillus species that produces 2,3-butanediol (2,3-BD), termed BRC1, was newly isolated, and a 2,3-BD dehydrogenase (Bdh) from this species was identified and characterized at the molecular and biochemical level. Sequence analysis revealed that Bdh is homologous to D-2,3-BD dehydrogenases. An analysis of the enzymatic properties of Bdh overexpressed in Escherichia coli confirmed the molecular results, showing preferred activity toward D-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Overexpression of bdh under the control of a xylose-inducible promoter resulted in increased enzyme activity and enhanced 2,3-BD production in Bacillus sp. BRC1. Additionally, a hydrolysate of cellulosic material, (empty palm fruit bunches), was successfully used for the enhanced production of 2,3-BD in the recombinant Bacillus strain.