FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk
Coordination between functionally related adjacent tissues is essential during development. For example, formation of trunk neural crest cells (NCCs) is highly influenced by the adjacent mesoderm, but the molecular mechanism involved is not well understood. As part of this mechanism, fibroblast grow...
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Published in | The Journal of cell biology Vol. 194; no. 3; pp. 489 - 503 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Rockefeller University Press
08.08.2011
The Rockefeller University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Coordination between functionally related adjacent tissues is essential during development. For example, formation of trunk neural crest cells (NCCs) is highly influenced by the adjacent mesoderm, but the molecular mechanism involved is not well understood. As part of this mechanism, fibroblast growth factor (FGF) and retinoic acid (RA) mesodermal gradients control the onset of neurogenesis in the extending neural tube. In this paper, using gain- and loss-of-function experiments, we show that caudal FGF signaling prevents premature specification of NCCs and, consequently, premature epithelial-mesenchymal transition (EMT) to allow cell emigration. In contrast, rostrally generated RA promotes EMT of NCCs at somitic levels. Furthermore, we show that FGF and RA signaling control EMT in part through the modulation of elements of the bone morphogenetic protein and Wnt signaling pathways. These data establish a clear role for opposition of FGF and RA signaling in control of the timing of NCC EMT and emigration and, consequently, coordination of the development of the central and peripheral nervous system during vertebrate trunk elongation. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 I. Olivera-Martínez’s present address is Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75252 Paris, Cedex 05, France. P.L. Martínez-Morales and R. Diez del Corral contributed equally to this paper. |
ISSN: | 0021-9525 1540-8140 |
DOI: | 10.1083/jcb.201011077 |