Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot
Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases ( n = 20), the S2 recombinant fragment based WB was po...
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Published in | Clinical immunology (Orlando, Fla.) Vol. 113; no. 2; pp. 145 - 150 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
01.11.2004
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases (
n = 20), the S2 recombinant fragment based WB was positive and this was comparable to the commercial ELISA using heat killed SARS-CoV. WB using the other four recombinant fragments in confirmed SARS cases generated lower rates of detection (S1—75%, S1-N—25%, S1-C—55%). Evaluation of sera from healthy controls (
n = 60) resulted in two weakly positive ELISA results with the remainder being negative while the S2 protein WB demonstrated three positive results from the 20 controls with a history of SARS contact and no positive results in 40 noncontact controls. A discrepancy between the ELISA and S2 WB arose when evaluating per-2003 sera from individuals (
n = 10) with SARS-like symptoms (ELISA—100% positive, S2 WB—30% positive). These data suggest that the S2 WB assay may be particularly useful in ELISA-negative SARS cases and in some ELISA-positive non-SARS cases. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this paper. |
ISSN: | 1521-6616 1521-7035 |
DOI: | 10.1016/j.clim.2004.07.003 |