Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot

Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases ( n = 20), the S2 recombinant fragment based WB was po...

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Published inClinical immunology (Orlando, Fla.) Vol. 113; no. 2; pp. 145 - 150
Main Authors Wang, Yue-Dan, Li, Yan, Xu, Guo-Bin, Dong, Xue-Yuan, Yang, Xiao-Ang, Feng, Zhen-Ru, Tian, Chan, Chen, Wei Feng
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 01.11.2004
Elsevier
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Summary:Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases ( n = 20), the S2 recombinant fragment based WB was positive and this was comparable to the commercial ELISA using heat killed SARS-CoV. WB using the other four recombinant fragments in confirmed SARS cases generated lower rates of detection (S1—75%, S1-N—25%, S1-C—55%). Evaluation of sera from healthy controls ( n = 60) resulted in two weakly positive ELISA results with the remainder being negative while the S2 protein WB demonstrated three positive results from the 20 controls with a history of SARS contact and no positive results in 40 noncontact controls. A discrepancy between the ELISA and S2 WB arose when evaluating per-2003 sera from individuals ( n = 10) with SARS-like symptoms (ELISA—100% positive, S2 WB—30% positive). These data suggest that the S2 WB assay may be particularly useful in ELISA-negative SARS cases and in some ELISA-positive non-SARS cases.
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These authors contributed equally to this paper.
ISSN:1521-6616
1521-7035
DOI:10.1016/j.clim.2004.07.003