The murine interleukin-4 receptor: Molecular cloning and characterization of secreted and membrane bound forms

• Published in: Cell Vol. 59; no. 2; pp. 335 - 348
• Main Authors: Mosley, Bruce, Beckmann, M.Patricia, March, Carl J., Idzerda, Rejean L., Gimpel, Steven D., VandenBos, Tim, Friend, Della, Alpert, Alan, Anderson, Dirk, Jackson, Jana, Wignall, Janis M., Smith, Craig, Gallis, Byron, Sims, John E., Urdal, David, Widmer, Michael B., Cosman, David, Park, Linda S.
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 20.10.1989; Cell Press
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cell Line; Cell Membrane - immunology; Cloning, Molecular - methods; DNA - genetics; DNA - isolation & purification; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Gene expression; genes; Interleukin-4 - metabolism; Kinetics; lymphocytes T; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Molecular Weight; Receptors, Interleukin-4; Receptors, Mitogen - biosynthesis; Receptors, Mitogen - genetics; Receptors, Mitogen - metabolism; Recombinant Proteins - metabolism; Restriction Mapping; T-Lymphocytes - immunology; Flow cytometry; Molecular hybridization; Purification; Rodentia; Cytokine; Primary structure; Characterization; Vertebrata; Interleukin 4; Mammalia; Cell line; Complementary DNA; Mouse; Affinity chromatography; Molecular cloning; Biological receptor
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/0092-8674(89)90295-X

Receptors for interleukin-4 (IL-4) are expressed at low levels on a wide variety of primary cells and cultured cell lines. Fluorescence-activated sorting of CTLL-2 cells resulted in the isolation of a subclone, CTLL 19.4, which expressed 10 6 IL-4 receptors per cell. These cells were used for the purification of IL-4 receptor protein and to prepare a hybrid-subtracted cDNA probe for isolation of cDNA clones. Three classes of IL-4 receptor cDNA were identified. The first encoded a 140 kd membrane bound IL-4 receptor containing extracellular, transmembrane, and cytoplasmic domains. The second class lacked the cytoplasmic region, and the third encoded a secreted form of the receptor. All cDNA clones expressed in COS-7 cells had IL-4 binding properties comparable to the native IL-4 receptor. The soluble form of the IL-4 receptor blocked the ability of IL-4 to induce CTLL cell proliferation and may represent a regulatory molecule specific for IL-4-dependent immune responses.