Changes in GnRH I, bradykinin and their receptors and GnIH in the ovary of Calotes versicolor during reproductive cycle

• Published in: General and comparative endocrinology Vol. 159; no. 2; pp. 158 - 169
• Main Authors: Singh, Padmasana, Krishna, Amitabh, Sridaran, Rajagopala, Tsutsui, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2008
• Subjects: Animals; Bradykinin; Bradykinin - metabolism; Bradykinin B 2 receptor; Calotes versicolor; Endocrinology & Metabolism; Female; GnIH; GnRH I; GnRH I receptor; Gonadotropin-Releasing Hormone - metabolism; Lizards - physiology; Ovary; Ovary - cytology; Ovary - metabolism; Receptors, Bradykinin - metabolism; Receptors, LHRH - metabolism; Reproduction - physiology; Reproductive cycle; Time Factors; Ovary; Calotes versicolor; Bradykinin; GnIH; GnRH I; Bradykinin B 2 receptor; GnRH I receptor; Reproductive cycle
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2008.08.016

Abstract The aim of this study was to investigate changes in the abundance of gonadotrophin releasing hormone I (GnRH I) and GnRH I receptor in the ovary of Calotes versicolor during the reproductive cycle and correlate them with the changes in gonadotrophin inhibitory hormone (GnIH), bradykinin and bradykinin B2 receptor in order to understand their interaction during ovarian cycle. GnRH I, bradykinin and their receptors and GnIH, were localized immunohistochemically in the ovary. Relative intensity of these peptides was estimated from the contralateral ovary using slot/Western blot followed by densitometry. The immunostaining of GnRH I, bradykinin and their receptors and GnIH were localized in the granulosa cells of previtellogenic follicles and stroma cells, whereas in the peripheral part of the cytoplasm in oocytes of vitellogenic and ovulatory follicles. The GnRH I immunostaining was relatively higher in inactive phase, but was low during active preovulatory phase suggesting inverse correlation with circulating estradiol level. The study showed a positive correlation between the expression pattern of GnRH I and GnIH, but showed a negative correlation between GnIH with GnRH I receptor in the ovary. This study further suggests a possibility for bradykinin regulating GnRH I synthesis in the ovary. An increase in the immunostaining of both GnRH I and GnIH in the oocyte prior to ovulation suggests their involvement in the oocyte maturation. It is thus concluded that the ovary of C. versicolor possesses GnRH I–GnIH–bradykinin system and interaction between these neuropeptides may be involved in the regulation of follicular development and oocyte maturation.