An evaluation of the RIDASCREEN and IDEIA enzyme immunoassays and the RIDAQUICK immunochromatographic test for the detection of norovirus in faecal specimens

• Published in: Journal of clinical virology Vol. 49; no. 4; pp. 254 - 257
• Main Authors: Kirby, Andrew, Gurgel, Ricardo Q, Dove, Winifred, Vieira, Sarah Cristina F, Cunliffe, Nigel A, Cuevas, Luis E
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2010; Elsevier
• Subjects: Allergy and Immunology; Biological and medical sciences; Brazil; Caliciviridae Infections - diagnosis; Caliciviridae Infections - virology; Child; Child, Preschool; ELISA; Feces - virology; Fundamental and applied biological sciences. Psychology; Gastroenteritis - virology; Human viral diseases; Humans; Immunoassay - methods; Immunochromatographic; Infant; Infant, Newborn; Infectious Disease; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Norovirus; Norovirus - isolation & purification; Reagent Kits, Diagnostic; Sensitivity; Sensitivity and Specificity; Specificity; Techniques used in virology; Viral diseases; Virology; Virology - methods; Brazil; Sensitivity; Specificity; Norovirus; Immunochromatographic; ELISA; Virus; Caliciviridae; Feces; Detection; Enzyme immunoassay; ELISA assay
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2010.08.004

Abstract Background The detection of norovirus by ELISA and immunochromatographic methods may facilitate epidemiological studies into the global disease burden associated with norovirus gastroenteritis and provide a quick method of testing for norovirus infection. Objectives To evaluate the new RIDASCREEN norovirus ELISA (3rd generation) and RIDAQUICK norovirus immunochromatographic test on a collection of samples from Brazilian children with acute gastroenteritis, and compare them against the established 2nd generation IDEIA norovirus assay. Study design Reverse transcriptase PCR, the study reference standard, was used to test 726 specimens for the presence of norovirus. All 96 norovirus positive samples and a systematic selection of negative samples were tested by RIDASCREEN, RIDAQUICK and IDEIA norovirus tests. Results The sensitivity of RIDASCREEN for the detection of norovirus was 63% (95% CI: 53–72%) and RIDAQUICK 69% (95% CI: 58–78%); both were >98% specific. The IDEIA had a sensitivity of 45% (95% CI: 35–55%), significantly lower than RIDASCREEN and RIDAQUICK ( p ≤ 0.01). The sensitivity of RIDASCREEN and RIDAQUICK in detecting GII.4 noroviruses, the principal norovirus strain identified in community and nosocomial infection globally, was 78% and 88% respectively. Conclusion The norovirus RIDASCREEN test may be useful in epidemiological studies of norovirus infection and the norovirus RIDAQUICK test offers an accurate and rapid method of detecting norovirus infection.